5AFI

2.9A Structure of E. coli ribosome-EF-TU complex by cs-corrected cryo-EM

これはPDB形式変換不可エントリーです。

5AFI の概要

• エントリーDOI: 10.2210/pdb5afi/pdb
• EMDBエントリー: 2847
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (66 entities in total)
• 機能のキーワード: ribosome, translation, protein synthesis, decoding, elongation factor tu, trna, rna modification, antibiotic
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 59
• 化学式量合計: 2302707.49

構造登録者

Fischer, N.,Neumann, P.,Konevega, A.L.,Bock, L.V.,Ficner, R.,Rodnina, M.V.,Stark, H. (登録日: 2015-01-22, 公開日: 2015-03-11, 最終更新日: 2025-03-12)

主引用文献

Fischer, N.,Neumann, P.,Konevega, A.L.,Bock, L.V.,Ficner, R.,Rodnina, M.V.,Stark, H.
Structure of the E. coli ribosome-EF-Tu complex at <3 angstrom resolution by Cs-corrected cryo-EM.
Nature, 520:567-570, 2015

PubMed Abstract: Single particle electron cryomicroscopy (cryo-EM) has recently made significant progress in high-resolution structure determination of macromolecular complexes due to improvements in electron microscopic instrumentation and computational image analysis. However, cryo-EM structures can be highly non-uniform in local resolution and all structures available to date have been limited to resolutions above 3 Å. Here we present the cryo-EM structure of the 70S ribosome from Escherichia coli in complex with elongation factor Tu, aminoacyl-tRNA and the antibiotic kirromycin at 2.65-2.9 Å resolution using spherical aberration (Cs)-corrected cryo-EM. Overall, the cryo-EM reconstruction at 2.9 Å resolution is comparable to the best-resolved X-ray structure of the E. coli 70S ribosome (2.8 Å), but provides more detailed information (2.65 Å) at the functionally important ribosomal core. The cryo-EM map elucidates for the first time the structure of all 35 rRNA modifications in the bacterial ribosome, explaining their roles in fine-tuning ribosome structure and function and modulating the action of antibiotics. We also obtained atomic models for flexible parts of the ribosome such as ribosomal proteins L9 and L31. The refined cryo-EM-based model presents the currently most complete high-resolution structure of the E. coli ribosome, which demonstrates the power of cryo-EM in structure determination of large and dynamic macromolecular complexes.

PubMed: 25707802
DOI: 10.1038/nature14275

実験手法

ELECTRON MICROSCOPY (2.9 Å)