5ACN

STRUCTURE OF ACTIVATED ACONITASE. FORMATION OF THE (4FE-4S) CLUSTER IN THE CRYSTAL

5ACN の概要

• エントリーDOI: 10.2210/pdb5acn/pdb
• 分子名称: ACONITASE, SULFATE ION, FE3-S4 CLUSTER, ... (5 entities in total)
• 機能のキーワード: lyase(carbon-oxygen)
• 由来する生物種: Sus scrofa (pig)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 83357.03

構造登録者

Robbins, A.H.,Stout, C.D. (登録日: 1990-01-16, 公開日: 1990-07-15, 最終更新日: 2024-11-20)

主引用文献

Robbins, A.H.,Stout, C.D.
Structure of activated aconitase: formation of the [4Fe-4S] cluster in the crystal.
Proc.Natl.Acad.Sci.USA, 86:3639-3643, 1989

PubMed Abstract: The structure of activated pig heart aconitase [citrate(isocitrate) hydro-lyase, EC 4.2.1.3] containing a [4Fe-4S] cluster has been refined at 2.5-A resolution to a crystallographic residual of 18.2%. Comparison of this structure to the recently determined 2.1-A resolution structure of the inactive enzyme containing a [3Fe-4S] cluster, by difference Fourier analysis, shows that upon activation iron is inserted into the structure isomorphously. The common atoms of the [3Fe-4S] and [4Fe-4S] cores agree within 0.1 A; the three common cysteinyl S gamma ligand atoms agree within 0.25 A. The fourth ligand of the Fe inserted into the [3Fe-4S] cluster is a water or hydroxyl from solvent, consistent with the absence of a free cysteine ligand in the enzyme active site cleft and the isomorphism of the two structures. A water molecule occupies a similar site in the crystal structure of the inactive enzyme.

PubMed: 2726740
DOI: 10.1073/pnas.86.10.3639

実験手法

X-RAY DIFFRACTION (2.1 Å)