5ACI

X-ray Structure of LPMO

5ACI の概要

• エントリーDOI: 10.2210/pdb5aci/pdb
• 関連するPDBエントリー: 5ACF 5ACG 5ACH 5ACJ
• 関連するBIRD辞書のPRD_ID: PRD_900020
• 分子名称: LYTIC POLYSACCHARIDE MONOOXYGENASE, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, COPPER (II) ION, ... (6 entities in total)
• 機能のキーワード: oxidoreductase, monooxygenase
• 由来する生物種: LENTINUS SIMILIS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26796.63

構造登録者

Frandsen, K.E.H.,Poulsen, J.N.,Tovborg, M.,Johanson, K.S.,Lo Leggio, L. (登録日: 2015-08-17, 公開日: 2016-03-02, 最終更新日: 2024-01-10)

主引用文献

Frandsen, K.E.,Simmons, T.J.,Dupree, P.,Poulsen, J.C.,Hemsworth, G.R.,Ciano, L.,Johnston, E.M.,Tovborg, M.,Johansen, K.S.,von Freiesleben, P.,Marmuse, L.,Fort, S.,Cottaz, S.,Driguez, H.,Henrissat, B.,Lenfant, N.,Tuna, F.,Baldansuren, A.,Davies, G.J.,Lo Leggio, L.,Walton, P.H.
The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases.
Nat. Chem. Biol., 12:298-303, 2016

PubMed Abstract: Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that oxidatively break down recalcitrant polysaccharides such as cellulose and chitin. Since their discovery, LPMOs have become integral factors in the industrial utilization of biomass, especially in the sustainable generation of cellulosic bioethanol. We report here a structural determination of an LPMO-oligosaccharide complex, yielding detailed insights into the mechanism of action of these enzymes. Using a combination of structure and electron paramagnetic resonance spectroscopy, we reveal the means by which LPMOs interact with saccharide substrates. We further uncover electronic and structural features of the enzyme active site, showing how LPMOs orchestrate the reaction of oxygen with polysaccharide chains.

PubMed: 26928935
DOI: 10.1038/nchembio.2029

実験手法

X-RAY DIFFRACTION (1.75 Å)