5ABV

Complex of D. melanogaster eIF4E with the 4E-binding protein Mextli

5ABV の概要

• エントリーDOI: 10.2210/pdb5abv/pdb
• 関連するPDBエントリー: 5ABU 5ABX 5ABY
• 分子名称: EUKARYOTIC TRANSLATION INITIATION FACTOR 4E, GH11071P (3 entities in total)
• 機能のキーワード: translation, gene regulation, cap binding protein, 4e binding protein
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 117897.05

構造登録者

Peter, D.,Weichenrieder, O. (登録日: 2015-08-09, 公開日: 2015-09-02, 最終更新日: 2024-01-10)

主引用文献

Peter, D.,Weber, R.,Koene, C.,Chung, M.-Y.,Ebertsch, L.,Truffault, V.,Weichenrieder, O.,Igreja, C.,Izaurralde, E.
Mextli Proteins Use Both Canonical Bipartite and Novel Tripartite Binding Modes to Form Eif4E Complexes that Display Differential Sensitivity to 4E-BP Regulation
Genes Dev., 29:1835-, 2015

PubMed Abstract: The eIF4E-binding proteins (4E-BPs) are a diverse class of translation regulators that share a canonical eIF4E-binding motif (4E-BM) with eIF4G. Consequently, they compete with eIF4G for binding to eIF4E, thereby inhibiting translation initiation. Mextli (Mxt) is an unusual 4E-BP that promotes translation by also interacting with eIF3. Here we present the crystal structures of the eIF4E-binding regions of the Drosophila melanogaster (Dm) and Caenorhabditis elegans (Ce) Mxt proteins in complex with eIF4E in the cap-bound and cap-free states. The structures reveal unexpected evolutionary plasticity in the eIF4E-binding mode, with a classical bipartite interface for Ce Mxt and a novel tripartite interface for Dm Mxt. Both interfaces comprise a canonical helix and a noncanonical helix that engage the dorsal and lateral surfaces of eIF4E, respectively. Remarkably, Dm Mxt contains a C-terminal auxiliary helix that lies anti-parallel to the canonical helix on the eIF4E dorsal surface. In contrast to the eIF4G and Ce Mxt complexes, the Dm eIF4E-Mxt complexes are resistant to competition by bipartite 4E-BPs, suggesting that Dm Mxt can bind eIF4E when eIF4G binding is inhibited. Our results uncovered unexpected diversity in the binding modes of 4E-BPs, resulting in eIF4E complexes that display differential sensitivity to 4E-BP regulation.

PubMed: 26294658
DOI: 10.1101/GAD.269068.115

実験手法

X-RAY DIFFRACTION (2.13 Å)