5AAQ

TBK1 recruitment to cytosol-invading Salmonella induces anti- bacterial autophagy

5AAQ の概要

• エントリーDOI: 10.2210/pdb5aaq/pdb
• 関連するPDBエントリー: 5AAS 5AAY 5AAZ
• NMR情報: BMRB: 25734
• 分子名称: CALCIUM-BINDING AND COILED-COIL DOMAIN-CONTAINING PROTEIN 2, ZINC ION (2 entities in total)
• 機能のキーワード: calcium-binding protein, tbk1, ndp52, zinc-finger
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6866.30

構造登録者

Thurston, T.L.,Allen, M.D.,Ravenhill, B.,Karpiyevitch, M.,Bloor, S.,Kaul, A.,Matthews, S.,Komander, D.,Holden, D.,Bycroft, M.,Randow, F. (登録日: 2015-07-28, 公開日: 2016-07-13, 最終更新日: 2024-05-15)

主引用文献

Thurston, T.L.,Boyle, K.B.,Allen, M.,Ravenhill, B.J.,Karpiyevich, M.,Bloor, S.,Kaul, A.,Noad, J.,Foeglein, A.,Matthews, S.A.,Komander, D.,Bycroft, M.,Randow, F.
Recruitment of Tbk1 to Cytosol-Invading Salmonella Induces Wipi2-Dependent Antibacterial Autophagy.
Embo J., 35:1779-, 2016

PubMed Abstract: Mammalian cells deploy autophagy to defend their cytosol against bacterial invaders. Anti-bacterial autophagy relies on the core autophagy machinery, cargo receptors, and "eat-me" signals such as galectin-8 and ubiquitin that label bacteria as autophagy cargo. Anti-bacterial autophagy also requires the kinase TBK1, whose role in autophagy has remained enigmatic. Here we show that recruitment of WIPI2, itself essential for anti-bacterial autophagy, is dependent on the localization of catalytically active TBK1 to the vicinity of cytosolic bacteria. Experimental manipulation of TBK1 recruitment revealed that engagement of TBK1 with any of a variety of Salmonella-associated "eat-me" signals, including host-derived glycans and K48- and K63-linked ubiquitin chains, suffices to restrict bacterial proliferation. Promiscuity in recruiting TBK1 via independent signals may buffer TBK1 functionality from potential bacterial antagonism and thus be of evolutionary advantage to the host.

PubMed: 27370208
DOI: 10.15252/EMBJ.201694491

実験手法

SOLUTION NMR