5A79

Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by cryo-EM

5A79 の概要

• エントリーDOI: 10.2210/pdb5a79/pdb
• 関連するPDBエントリー: 5A7A
• EMDBエントリー: 3073
• 分子名称: CAPSID PROTEIN, RNA (2 entities in total)
• 機能のキーワード: bsmv, virus, helical, cryo-em, image processing, msa
• 由来する生物種: BARLEY STRIPE MOSAIC VIRUS; 詳細
• 細胞内の位置: Virion : P04866
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23513.88

構造登録者

Clare, D.K.,Pechnikova, E.,Skurat, E.,Makarov, V.,Sokolova, O.S.,Solovyev, A.G.,V Orlova, E. (登録日: 2015-07-03, 公開日: 2015-09-02, 最終更新日: 2024-05-08)

主引用文献

Clare, D.K.,Pechnikova, E.V.,Skurat, E.V.,Makarov, V.V.,Sokolova, O.S.,Solovyev, A.G.,Orlova, E.V.
Novel Inter-Subunit Contacts in Barley Stripe Mosaic Virus Revealed by Cryo-Electron Microscopy.
Structure, 23:1815-, 2015

PubMed Abstract: Barley stripe mosaic virus (BSMV, genus Hordeivirus) is a rod-shaped single-stranded RNA virus similar to viruses of the structurally characterized and well-studied genus Tobamovirus. Here we report the first high-resolution structure of BSMV at 4.1 Å obtained by cryo-electron microscopy. We discovered that BSMV forms two types of virion that differ in the number of coat protein (CP) subunits per turn and interactions between the CP subunits. While BSMV and tobacco mosaic virus CP subunits have a similar fold and interact with RNA using conserved residues, the axial contacts between the CP of these two viral groups are considerably different. BSMV CP subunits lack substantial axial contacts and are held together by a previously unobserved lateral contact formed at the virion surface via an interacting loop, which protrudes from the CP hydrophobic core to the adjacent CP subunit. These data provide an insight into diversity in structural organization of helical viruses.

PubMed: 26278173
DOI: 10.1016/J.STR.2015.06.028

実験手法

ELECTRON MICROSCOPY (4.1 Å)