5A6P

Heavy metal associated domain of NLR-type immune receptor Pikp1 from rice (Oryza sativa)

5A6P の概要

• エントリーDOI: 10.2210/pdb5a6p/pdb
• 関連するPDBエントリー: 5A6W
• 分子名称: RESISTANCE PROTEIN PIKP-1 (2 entities in total)
• 機能のキーワード: immune system, heavy metal associated domain, nlr immune receptor
• 由来する生物種: ORYZA SATIVA (RICE)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15632.46

構造登録者

Maqbool, A.,Saitoh, H.,Franceschetti, M.,Stevenson, C.E.,Uemura, A.,Kanzaki, H.,Kamoun, S.,Terauchi, R.,Banfield, M.J. (登録日: 2015-06-30, 公開日: 2015-08-26, 最終更新日: 2024-05-08)

主引用文献

Maqbool, A.,Saitoh, H.,Franceschetti, M.,Stevenson, C.E.,Uemura, A.,Kanzaki, H.,Kamoun, S.,Terauchi, R.,Banfield, M.J.
Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor.
Elife, 4:-, 2015

PubMed Abstract: Plants have evolved intracellular immune receptors to detect pathogen proteins known as effectors. How these immune receptors detect effectors remains poorly understood. Here we describe the structural basis for direct recognition of AVR-Pik, an effector from the rice blast pathogen, by the rice intracellular NLR immune receptor Pik. AVR-PikD binds a dimer of the Pikp-1 HMA integrated domain with nanomolar affinity. The crystal structure of the Pikp-HMA/AVR-PikD complex enabled design of mutations to alter protein interaction in yeast and in vitro, and perturb effector-mediated response both in a rice cultivar containing Pikp and upon expression of AVR-PikD and Pikp in the model plant Nicotiana benthamiana. These data reveal the molecular details of a recognition event, mediated by a novel integrated domain in an NLR, which initiates a plant immune response and resistance to rice blast disease. Such studies underpin novel opportunities for engineering disease resistance to plant pathogens in staple food crops.

PubMed: 26304198
DOI: 10.7554/eLife.08709

実験手法

X-RAY DIFFRACTION (2.1 Å)