5A6G

Cryo-EM structure of the Slo2.2 Na-activated K channel

5A6G の概要

• エントリーDOI: 10.2210/pdb5a6g/pdb
• 関連するPDBエントリー: 5A6F 5A6G
• EMDBエントリー: 3064
• 分子名称: S1-S4 DOMAIN OF POTASSIUM CHANNEL SUBFAMILY T MEMBER 1, PORE DOMAIN OF POTASSIUM CHANNEL SUBFAMILY T MEMBER 1 (2 entities in total)
• 機能のキーワード: transport, ion channel, potassium channel
• 由来する生物種: GALLUS GALLUS (CHICKEN); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22563.49

構造登録者

Hite, R.K.,Yuan, P.,Li, Z.,Hsuing, Y.,Walz, T.,MacKinnon, R. (登録日: 2015-06-25, 公開日: 2015-10-14, 最終更新日: 2024-05-08)

主引用文献

Hite, R.K.,Yuan, P.,Li, Z.,Hsuing, Y.,Walz, T.,Mackinnon, R.
Cryo-Electron Microscopy Structure of the Slo2.2 Na1-Activated K1 Channel
Nature, 527:198-, 2015

PubMed Abstract: Na(+)-activated K(+) channels are members of the Slo family of large conductance K(+) channels that are widely expressed in the brain, where their opening regulates neuronal excitability. These channels fulfil a number of biological roles and have intriguing biophysical properties, including conductance levels that are ten times those of most other K(+) channels and gating sensitivity to intracellular Na(+). Here we present the structure of a complete Na(+)-activated K(+) channel, chicken Slo2.2, in the Na(+)-free state, determined by cryo-electron microscopy at a nominal resolution of 4.5 ångströms. The channel is composed of a large cytoplasmic gating ring, in which resides the Na(+)-binding site and a transmembrane domain that closely resembles voltage-gated K(+) channels. In the structure, the cytoplasmic domain adopts a closed conformation and the ion conduction pore is also closed. The structure reveals features that can explain the unusually high conductance of Slo channels and how contraction of the cytoplasmic gating ring closes the pore.

PubMed: 26436452
DOI: 10.1038/NATURE14958

実験手法

ELECTRON MICROSCOPY (5.2 Å)