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5A6F

Cryo-EM structure of the Slo2.2 Na-activated K channel

Summary for 5A6F
Entry DOI10.2210/pdb5a6f/pdb
Related5A6E 5A6G
EMDB information3063
DescriptorGATING RING OF POTASSIUM CHANNEL SUBFAMILY T MEMBER 1, RCK2 ELABORATION OF POTASSIUM CHANNEL SUBFAMILY T MEMBER 1 (2 entities in total)
Functional Keywordstransport, ion channel, potassium channel
Biological sourceGALLUS GALLUS (CHICKEN)
More
Total number of polymer chains2
Total formula weight82998.57
Authors
Hite, R.K.,Yuan, P.,Li, Z.,Hsuing, Y.,Walz, T.,MacKinnon, R. (deposition date: 2015-06-25, release date: 2015-10-14, Last modification date: 2024-05-08)
Primary citationHite, R.K.,Yuan, P.,Li, Z.,Hsuing, Y.,Walz, T.,Mackinnon, R.
Cryo-Electron Microscopy Structure of the Slo2.2 Na1-Activated K1 Channel
Nature, 527:198-, 2015
Cited by
PubMed Abstract: Na(+)-activated K(+) channels are members of the Slo family of large conductance K(+) channels that are widely expressed in the brain, where their opening regulates neuronal excitability. These channels fulfil a number of biological roles and have intriguing biophysical properties, including conductance levels that are ten times those of most other K(+) channels and gating sensitivity to intracellular Na(+). Here we present the structure of a complete Na(+)-activated K(+) channel, chicken Slo2.2, in the Na(+)-free state, determined by cryo-electron microscopy at a nominal resolution of 4.5 ångströms. The channel is composed of a large cytoplasmic gating ring, in which resides the Na(+)-binding site and a transmembrane domain that closely resembles voltage-gated K(+) channels. In the structure, the cytoplasmic domain adopts a closed conformation and the ion conduction pore is also closed. The structure reveals features that can explain the unusually high conductance of Slo channels and how contraction of the cytoplasmic gating ring closes the pore.
PubMed: 26436452
DOI: 10.1038/NATURE14958
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (4.2 Å)
Structure validation

239803

数据于2025-08-06公开中

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