5A6C
Concomitant binding of Afadin to LGN and F-actin directs planar spindle orientation
5A6C の概要
エントリーDOI | 10.2210/pdb5a6c/pdb |
分子名称 | G-PROTEIN-SIGNALING MODULATOR 2, AFADIN, SULFATE ION (2 entities in total) |
機能のキーワード | cell adhesion, mitotic spindle orientation, lgn, afadin |
由来する生物種 | HOMO SAPIENS (HUMAN) |
細胞内の位置 | Cell junction, adherens junction: P55196 |
タンパク質・核酸の鎖数 | 2 |
化学式量合計 | 83288.40 |
構造登録者 | Carminati, M.,Gallini, S.,Pirovano, L.,Alfieri, A.,Bisi, S.,Mapelli, M. (登録日: 2015-06-25, 公開日: 2015-12-30, 最終更新日: 2024-01-10) |
主引用文献 | Carminati, M.,Gallini, S.,Pirovano, L.,Alfieri, A.,Bisi, S.,Mapelli, M. Concomitant Binding of Afadin to Lgn and F-Actin Directs Planar Spindle Orientation. Nat.Struct.Mol.Biol., 23:155-, 2016 Cited by PubMed Abstract: Polarized epithelia form by oriented cell divisions in which the mitotic spindle aligns parallel to the epithelial plane. To orient the mitotic spindle, cortical cues trigger the recruitment of NuMA-dynein-based motors, which pull on astral microtubules via the protein LGN. We demonstrate that the junctional protein Afadin is required for spindle orientation and correct epithelial morphogenesis of Caco-2 cysts. Molecularly, Afadin binds directly and concomitantly to F-actin and to LGN. We determined the crystallographic structure of human Afadin in complex with LGN and show that it resembles the LGN-NuMA complex. In mitosis, Afadin is necessary for cortical accumulation of LGN and NuMA above the spindle poles, in an F-actin-dependent manner. Collectively, our results depict Afadin as a molecular hub governing the enrichment of LGN and NuMA at the cortex. To our knowledge, Afadin is the first-described mechanical anchor between dynein and cortical F-actin. PubMed: 26751642DOI: 10.1038/NSMB.3152 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.901 Å) |
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