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5A6C

Concomitant binding of Afadin to LGN and F-actin directs planar spindle orientation

5A6C の概要
エントリーDOI10.2210/pdb5a6c/pdb
分子名称G-PROTEIN-SIGNALING MODULATOR 2, AFADIN, SULFATE ION (2 entities in total)
機能のキーワードcell adhesion, mitotic spindle orientation, lgn, afadin
由来する生物種HOMO SAPIENS (HUMAN)
細胞内の位置Cell junction, adherens junction: P55196
タンパク質・核酸の鎖数2
化学式量合計83288.40
構造登録者
Carminati, M.,Gallini, S.,Pirovano, L.,Alfieri, A.,Bisi, S.,Mapelli, M. (登録日: 2015-06-25, 公開日: 2015-12-30, 最終更新日: 2024-01-10)
主引用文献Carminati, M.,Gallini, S.,Pirovano, L.,Alfieri, A.,Bisi, S.,Mapelli, M.
Concomitant Binding of Afadin to Lgn and F-Actin Directs Planar Spindle Orientation.
Nat.Struct.Mol.Biol., 23:155-, 2016
Cited by
PubMed Abstract: Polarized epithelia form by oriented cell divisions in which the mitotic spindle aligns parallel to the epithelial plane. To orient the mitotic spindle, cortical cues trigger the recruitment of NuMA-dynein-based motors, which pull on astral microtubules via the protein LGN. We demonstrate that the junctional protein Afadin is required for spindle orientation and correct epithelial morphogenesis of Caco-2 cysts. Molecularly, Afadin binds directly and concomitantly to F-actin and to LGN. We determined the crystallographic structure of human Afadin in complex with LGN and show that it resembles the LGN-NuMA complex. In mitosis, Afadin is necessary for cortical accumulation of LGN and NuMA above the spindle poles, in an F-actin-dependent manner. Collectively, our results depict Afadin as a molecular hub governing the enrichment of LGN and NuMA at the cortex. To our knowledge, Afadin is the first-described mechanical anchor between dynein and cortical F-actin.
PubMed: 26751642
DOI: 10.1038/NSMB.3152
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.901 Å)
構造検証レポート
Validation report summary of 5a6c
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-08-06に公開中

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