5A5C

Structure of an engineered neuronal LRRTM2 adhesion molecule

5A5C の概要

• エントリーDOI: 10.2210/pdb5a5c/pdb
• 分子名称: LRRTM (2 entities in total)
• 機能のキーワード: signaling protein, lrrtm, synapse, adhesion, leucine rich repeat, neurexin
• 由来する生物種: SYNTHETIC CONSTRUCT
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 160185.56

構造登録者

Paatero, A.,Rosti, K.,Shkumatov, A.V.,Brunello, C.,Kysenius, K.,Huttunen, H.,Kajander, T. (登録日: 2015-06-17, 公開日: 2016-02-03, 最終更新日: 2024-11-13)

主引用文献

Paatero, A.,Rosti, K.,Shkumatov, A.V.,Sele, C.,Brunello, C.,Kysenius, K.,Singha, P.,Jokinen, V.,Huttunen, H.,Kajander, T.
Crystal Structure of an Engineered Lrrtm2 Synaptic Adhesion Molecule and a Model for Neurexin Binding.
Biochemistry, 55:914-, 2016

PubMed Abstract: Synaptic adhesion molecules are key components in development of the brain, and in the formation of neuronal circuits, as they are central in the assembly and maturation of chemical synapses. Several families of neuronal adhesion molecules have been identified such as the neuronal cell adhesion molecules, neurexins and neuroligins, and in particular recently several leucine-rich repeat proteins, e.g., Netrin G-ligands, SLITRKs, and LRRTMs. The LRRTMs form a family of four proteins. They have been implicated in excitatory glutamatergic synapse function and were specifically characterized as ligands for neurexins in excitatory synapse formation and maintenance. In addition, LRRTM3 and LRRTM4 have been found to be ligands for heparan sulfate proteoglycans, including glypican. We report here the crystal structure of a thermostabilized mouse LRRTM2, with a Tm 30 °C higher than that of the wild-type protein. We localized the neurexin binding site to the concave surface based on protein engineering, sequence conservation, and prior information about the interaction of the ligand with neurexins, which allowed us to propose a tentative model for the LRRTM-neurexin interaction complex. We also determined affinities of the thermostabilized LRRTM2 and wild-type LRRTM1 and LRRTM2 for neurexin-β1 with and without Ca(2+). Cell culture studies and binding experiments show that the engineered protein is functional and capable of forming synapselike contacts. The structural and functional data presented here provide the first structure of an LRRTM protein and allow us to propose a model for the molecular mechanism of LRRTM function in the synaptic adhesion.

PubMed: 26785044
DOI: 10.1021/ACS.BIOCHEM.5B00971

実験手法

X-RAY DIFFRACTION (2.097 Å)