5A45

Structure of Bacteriorhodopsin obtained from 5um crystals by multi crystal data collection

5A45 の概要

• エントリーDOI: 10.2210/pdb5a45/pdb
• 関連するPDBエントリー: 5A3Y 5A3Z 5A44 5A47
• 分子名称: BACTERIORHODOPSIN, RETINAL (2 entities in total)
• 機能のキーワード: proton transport, multi crystal data collection, synchrotron serial crystallography, membrane protein, lcp, ssx, ion pump, retinal protein, photoreceptor, 7-helix transmembrane, ion transport
• 由来する生物種: HALOBACTERIUM SALINARUM
• 細胞内の位置: Cell membrane; Multi-pass membrane protein: P02945
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27098.85

構造登録者

Zander, U.,Bourenkov, G.,Popov, A.N.,de Sanctis, D.,McCarthy, A.A.,Svensson, O.,Round, E.S.,Gordeliy, V.I.,Mueller-Dieckmann, C.,Leonard, G.A. (登録日: 2015-06-05, 公開日: 2015-11-11, 最終更新日: 2024-11-20)

主引用文献

Zander, U.,Bourenkov, G.,Popov, A.N.,De Sanctis, D.,Svensson, O.,Mccarthy, A.A.,Round, E.,Gordeliy, V.,Mueller-Dieckmann, C.,Leonard, G.A.
Meshandcollect: An Automated Multi-Crystal Data-Collection Workflow for Synchrotron Macromolecular Crystallography Beamlines.
Acta Crystallogr.,Sect.D, 71:2328-, 2015

PubMed Abstract: Here, an automated procedure is described to identify the positions of many cryocooled crystals mounted on the same sample holder, to rapidly predict and rank their relative diffraction strengths and to collect partial X-ray diffraction data sets from as many of the crystals as desired. Subsequent hierarchical cluster analysis then allows the best combination of partial data sets, optimizing the quality of the final data set obtained. The results of applying the method developed to various systems and scenarios including the compilation of a complete data set from tiny crystals of the membrane protein bacteriorhodopsin and the collection of data sets for successful structure determination using the single-wavelength anomalous dispersion technique are also presented.

PubMed: 26527148
DOI: 10.1107/S1399004715017927

実験手法

X-RAY DIFFRACTION (2.57 Å)