5A44

Structure of Bacteriorhodopsin obtained from 20um crystals by multi crystal data collection

Summary for 5A44

• Entry DOI: 10.2210/pdb5a44/pdb
• Related: 5A3Y 5A3Z 5A45 5A47
• Descriptor: BACTERIORHODOPSIN, RETINAL, 1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL, ... (5 entities in total)
• Functional Keywords: proton transport, multi crystal data collection, synchrotron serial crystallography, lcp, ssx, ion pump, membrane protein, retinal protein, photoreceptor, 7-helix transmembrane, ion transport, membrane
• Biological source: HALOBACTERIUM SALINARUM
• Cellular location: Cell membrane; Multi-pass membrane protein: P02945
• Total number of polymer chains: 1
• Total formula weight: 32951.22

Authors

Zander, U.,Bourenkov, G.,Popov, A.N.,de Sanctis, D.,McCarthy, A.A.,Svensson, O.,Round, E.S.,Gordeliy, V.I.,Mueller-Dieckmann, C.,Leonard, G.A. (deposition date: 2015-06-05, release date: 2015-11-11, Last modification date: 2024-11-13)

Primary citation

Zander, U.,Bourenkov, G.,Popov, A.N.,De Sanctis, D.,Svensson, O.,Mccarthy, A.A.,Round, E.,Gordeliy, V.,Mueller-Dieckmann, C.,Leonard, G.A.
Meshandcollect: An Automated Multi-Crystal Data-Collection Workflow for Synchrotron Macromolecular Crystallography Beamlines.
Acta Crystallogr.,Sect.D, 71:2328-, 2015

PubMed Abstract: Here, an automated procedure is described to identify the positions of many cryocooled crystals mounted on the same sample holder, to rapidly predict and rank their relative diffraction strengths and to collect partial X-ray diffraction data sets from as many of the crystals as desired. Subsequent hierarchical cluster analysis then allows the best combination of partial data sets, optimizing the quality of the final data set obtained. The results of applying the method developed to various systems and scenarios including the compilation of a complete data set from tiny crystals of the membrane protein bacteriorhodopsin and the collection of data sets for successful structure determination using the single-wavelength anomalous dispersion technique are also presented.

PubMed: 26527148
DOI: 10.1107/S1399004715017927

Experimental method

X-RAY DIFFRACTION (2.29 Å)