5A2G

An esterase from anaerobic Clostridium hathewayi can hydrolyze aliphatic aromatic polyesters

5A2G の概要

• エントリーDOI: 10.2210/pdb5a2g/pdb
• 分子名称: CARBOXYLIC ESTER HYDROLASE, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: hydrolase, microbial polyesterase, anaerobic esterase, polyester biodegradation, biogas batch, anaerobic biodegradation, clostridium hathewayi
• 由来する生物種: HUNGATELLA HATHEWAYI
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 233498.65

構造登録者

Hromic, A.,Pavkov Keller, T.,Steinkellner, G.,Gruber, K.,Perz, V.,Baumschlager, A.,Bleymaier, K.,Zitzenbacher, S.,Zankel, A.,Mayrhofer, C.,Sinkel, C.,Kueper, U.,Schlegel, K.A.,Ribitsch, D.,Guebitz, G.M. (登録日: 2015-05-19, 公開日: 2016-02-24, 最終更新日: 2024-01-10)

主引用文献

Perz, V.,Hromic, A.,Baumschlager, A.,Steinkellner, G.,Pavkov-Keller, T.,Gruber, K.,Bleymaier, K.,Zitzenbacher, S.,Zankel, A.,Mayrhofer, C.,Sinkel, C.,Kueper, U.,Schlegel, K.,Ribitsch, D.,Guebitz, G.M.
An Esterase from Anaerobic Clostridium Hathewayi Can Hydrolyze Aliphatic-Aromatic Polyesters.
Environ.Sci.Tech., 50:2899-, 2016

PubMed Abstract: Recently, a variety of biodegradable polymers have been developed as alternatives to recalcitrant materials. Although many studies on polyester biodegradability have focused on aerobic environments, there is much less known on biodegradation of polyesters in natural and artificial anaerobic habitats. Consequently, the potential of anaerobic biogas sludge to hydrolyze the synthetic compostable polyester PBAT (poly(butylene adipate-co-butylene terephthalate) was evaluated in this study. On the basis of reverse-phase high-performance liquid chromatography (RP-HPLC) analysis, accumulation of terephthalic acid (Ta) was observed in all anaerobic batches within the first 14 days. Thereafter, a decline of Ta was observed, which occurred presumably due to consumption by the microbial population. The esterase Chath_Est1 from the anaerobic risk 1 strain Clostridium hathewayi DSM-13479 was found to hydrolyze PBAT. Detailed characterization of this esterase including elucidation of the crystal structure was performed. The crystal structure indicates that Chath_Est1 belongs to the α/β-hydrolases family. This study gives a clear hint that also micro-organisms in anaerobic habitats can degrade manmade PBAT.

PubMed: 26878094
DOI: 10.1021/ACS.EST.5B04346

実験手法

X-RAY DIFFRACTION (1.899 Å)