5A18

The structure of the SOLE element of oskar mRNA

5A18 の概要

• エントリーDOI: 10.2210/pdb5a18/pdb
• 関連するPDBエントリー: 5A17
• NMR情報: BMRB: 26568
• 分子名称: OSKAR MRNA (1 entity in total)
• 機能のキーワード: rna, sole element, oskar
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10312.20

構造登録者

Simon, B.,Masiewicz, P.,Ephrussi, A.,Carlomagno, T. (登録日: 2015-04-28, 公開日: 2015-05-06, 最終更新日: 2024-06-19)

主引用文献

Simon, B.,Masiewicz, P.,Ephrussi, A.,Carlomagno, T.
The Structure of the Sole Element of Oskar Mrna.
RNA, 21:1444-1453, 2015

PubMed Abstract: mRNA localization by active transport is a regulated process that requires association of mRNPs with protein motors for transport along either the microtubule or the actin cytoskeleton. oskar mRNA localization at the posterior pole of the Drosophila oocyte requires a specific mRNA sequence, termed the SOLE, which comprises nucleotides of both exon 1 and exon 2 and is assembled upon splicing. The SOLE folds into a stem-loop structure. Both SOLE RNA and the exon junction complex (EJC) are required for oskar mRNA transport along the microtubules by kinesin. The SOLE RNA likely constitutes a recognition element for a yet unknown protein, which either belongs to the EJC or functions as a bridge between the EJC and the mRNA. Here, we determine the solution structure of the SOLE RNA by Nuclear Magnetic Resonance spectroscopy. We show that the SOLE forms a continuous helical structure, including a few noncanonical base pairs, capped by a pentanucleotide loop. The helix displays a widened major groove, which could accommodate a protein partner. In addition, the apical helical segment undergoes complex dynamics, with potential functional significance.

PubMed: 26089324
DOI: 10.1261/RNA.049601.115

実験手法

SOLUTION NMR