4ZTC

PglE Aminotransferase in complex with External Aldimine, Mutant K184A

4ZTC の概要

• エントリーDOI: 10.2210/pdb4ztc/pdb
• 分子名称: Aminotransferase homolog, [(2R,3R,4R,5S,6R)-3-acetamido-6-methyl-5-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-4-oxidanyl-oxan-2-yl] [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate (3 entities in total)
• 機能のキーワード: aminotransferase, n-n'-diacetylbacillosamine, n-glycosylation pyridoxyl 5'-phosphate, transferase
• 由来する生物種: Campylobacter jejuni
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44618.70

構造登録者

Riegert, A.S.,Thoden, J.B.,Young, N.M.,Watson, D.C.,Holden, H.M. (登録日: 2015-05-14, 公開日: 2015-07-29, 最終更新日: 2023-09-27)

主引用文献

Riegert, A.S.,Young, N.M.,Watson, D.C.,Thoden, J.B.,Holden, H.M.
Structure of the external aldimine form of PglE, an aminotransferase required for N,N'-diacetylbacillosamine biosynthesis.
Protein Sci., 24:1609-1616, 2015

PubMed Abstract: N,N'-diacetylbacillosamine is a novel sugar that plays a key role in bacterial glycosylation. Three enzymes are required for its biosynthesis in Campylobacter jejuni starting from UDP-GlcNAc. The focus of this investigation, PglE, catalyzes the second step in the pathway. It is a PLP-dependent aminotransferase that converts UDP-2-acetamido-4-keto-2,4,6-trideoxy-d-glucose to UDP-2-acetamido-4-amino-2,4,6-trideoxy-d-glucose. For this investigation, the structure of PglE in complex with an external aldimine was determined to a nominal resolution of 2.0 Å. A comparison of its structure with those of other sugar aminotransferases reveals a remarkable difference in the manner by which PglE accommodates its nucleotide-linked sugar substrate.

PubMed: 26178292
DOI: 10.1002/pro.2745

実験手法

X-RAY DIFFRACTION (2 Å)