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4ZRV

Structure of cow mincle CRD complexed with trehalose mono butyrate

4ZRV の概要
エントリーDOI10.2210/pdb4zrv/pdb
関連するPDBエントリー4ZRW
関連するBIRD辞書のPRD_IDPRD_900091
分子名称Mincle CRD, 6-O-butanoyl-alpha-D-glucopyranose-(1-1)-alpha-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
機能のキーワードglycobiology, carbohydrate-binding protein, c-type lectin, complex, sugar binding protein
由来する生物種Bos taurus (Bovine)
タンパク質・核酸の鎖数3
化学式量合計48997.16
構造登録者
Feinberg, H.,Rambaruth, N.D.S.,Taylor, M.E.,Drickamer, K.,Weis, W.I. (登録日: 2015-05-12, 公開日: 2016-05-25, 最終更新日: 2024-10-30)
主引用文献Feinberg, H.,Rambaruth, N.D.,Jegouzo, S.A.,Jacobsen, K.M.,Djurhuus, R.,Poulsen, T.B.,Weis, W.I.,Taylor, M.E.,Drickamer, K.
Binding Sites for Acylated Trehalose Analogs of Glycolipid Ligands on an Extended Carbohydrate Recognition Domain of the Macrophage Receptor Mincle.
J.Biol.Chem., 291:21222-21233, 2016
Cited by
PubMed Abstract: The macrophage receptor mincle binds to trehalose dimycolate on the surface of Mycobacterium tuberculosis Signaling initiated by this interaction leads to cytokine production, which underlies the ability of mycobacteria to evade the immune system and also to function as adjuvants. In previous work the mechanism for binding of the sugar headgroup of trehalose dimycolate to mincle has been elucidated, but the basis for enhanced binding to glycolipid ligands, in which hydrophobic substituents are attached to the 6-hydroxyl groups, has been the subject of speculation. In the work reported here, the interaction of trehalose derivatives with bovine mincle has been probed with a series of synthetic mimics of trehalose dimycolate in binding assays, in structural studies by x-ray crystallography, and by site-directed mutagenesis. Binding studies reveal that, rather than reflecting specific structural preference, the apparent affinity of mincle for ligands with hydrophobic substituents correlates with their overall size. Structural and mutagenesis analysis provides evidence for interaction of the hydrophobic substituents with multiple different portions of the surface of mincle and confirms the presence of three Ca-binding sites. The structure of an extended portion of the extracellular domain of mincle, beyond the minimal C-type carbohydrate recognition domain, also constrains the way the binding domains may interact on the surface of macrophages.
PubMed: 27542410
DOI: 10.1074/jbc.M116.749515
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.095 Å)
構造検証レポート
Validation report summary of 4zrv
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-23に公開中

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