4ZM1

Shigella flexneri lipopolysaccharide O-antigen chain-length regulator WzzBSF - wild type

4ZM1 の概要

• エントリーDOI: 10.2210/pdb4zm1/pdb
• 関連するPDBエントリー: 4ZM5
• 分子名称: Chain length determinant protein, CITRIC ACID, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: lipopolysaccharide, chain-length, virulence, serospecificity, membrane protein
• 由来する生物種: Shigella flexneri
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 82941.46

構造登録者

Ericsson, D.J.,Chang, C.-W.,Lonhienne, T.,Casey, L.,Benning, F.,Kobe, B.,Tran, E.N.H.,Morona, R. (登録日: 2015-05-02, 公開日: 2016-03-23, 最終更新日: 2023-09-27)

主引用文献

Chang, C.W.,Tran, E.N.,Ericsson, D.J.,Casey, L.W.,Lonhienne, T.,Benning, F.,Morona, R.,Kobe, B.
Structural and Biochemical Analysis of a Single Amino-Acid Mutant of WzzBSF That Alters Lipopolysaccharide O-Antigen Chain Length in Shigella flexneri.
Plos One, 10:e0138266-e0138266, 2015

PubMed Abstract: Lipopolysaccharide (LPS), a surface polymer of Gram-negative bacteria, helps bacteria survive in different environments and acts as a virulence determinant of host infection. The O-antigen (Oag) component of LPS exhibits a modal chain-length distribution that is controlled by polysaccharide co-polymerases (PCPs). The molecular basis of the regulation of Oag chain-lengths remains unclear, despite extensive mutagenesis and structural studies of PCPs from Escherichia coli and Shigella. Here, we identified a single mutation (A107P) of the Shigella flexneri WzzBSF, by a random mutagenesis approach, that causes a shortened Oag chain-length distribution in bacteria. We determined the crystal structures of the periplasmic domains of wild-type WzzBSF and the A107P mutant. Both structures form a highly similar open trimeric assembly in the crystals, and show a similar tendency to self-associate in solution. Binding studies by bio-layer interferometry reveal cooperative binding of very short (VS)-core-plus-O-antigen polysaccharide (COPS) to the periplasmic domains of both proteins, but with decreased affinity for the A107P mutant. Our studies reveal that subtle and localized structural differences in PCPs can have dramatic effects on LPS chain-length distribution in bacteria, for example by altering the affinity for the substrate, which supports the role of the structure of the growing Oag polymer in this process.

PubMed: 26378781
DOI: 10.1371/journal.pone.0138266

実験手法

X-RAY DIFFRACTION (2.55 Å)