4ZKX

Crystal structure of the PmFTN variant E44Q soaked in iron (5 min)

4ZKX の概要

• エントリーDOI: 10.2210/pdb4zkx/pdb
• 関連するPDBエントリー: 4ZKH 4ZKW 4ZL5 4ZL6
• 分子名称: Ferritin, FE (III) ION (3 entities in total)
• 機能のキーワード: ferritin, di-iron ferroxidase centre, 4-helix bundle, oxidoreductase
• 由来する生物種: Pseudo-nitzschia multiseries
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 152167.79

構造登録者

Pfaffen, S.,Murphy, M.E.P. (登録日: 2015-04-30, 公開日: 2015-09-30, 最終更新日: 2023-09-27)

主引用文献

Pfaffen, S.,Bradley, J.M.,Abdulqadir, R.,Firme, M.R.,Moore, G.R.,Le Brun, N.E.,Murphy, M.E.
A Diatom Ferritin Optimized for Iron Oxidation but Not Iron Storage.
J.Biol.Chem., 290:28416-28427, 2015

PubMed Abstract: Ferritin from the marine pennate diatom Pseudo-nitzschia multiseries (PmFTN) plays a key role in sustaining growth in iron-limited ocean environments. The di-iron catalytic ferroxidase center of PmFTN (sites A and B) has a nearby third iron site (site C) in an arrangement typically observed in prokaryotic ferritins. Here we demonstrate that Glu-44, a site C ligand, and Glu-130, a residue that bridges iron bound at sites B and C, limit the rate of post-oxidation reorganization of iron coordination and the rate at which Fe(3+) exits the ferroxidase center for storage within the mineral core. The latter, in particular, severely limits the overall rate of iron mineralization. Thus, the diatom ferritin is optimized for initial Fe(2+) oxidation but not for mineralization, pointing to a role for this protein in buffering iron availability and facilitating iron-sparing rather than only long-term iron storage.

PubMed: 26396187
DOI: 10.1074/jbc.M115.669713

実験手法

X-RAY DIFFRACTION (1.8 Å)