4ZKV

Crystal structure of human histidine triad nucleotide-binding protein 1 (hHINT1) refined to 1.92A at P21 space group

Summary for 4ZKV

• Entry DOI: 10.2210/pdb4zkv/pdb
• Related: 1KPC
• Descriptor: Histidine triad nucleotide-binding protein 1, SULFATE ION (3 entities in total)
• Functional Keywords: hint, hit, histidine triad, hydrolase, phosphoramidase
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm: P49773
• Total number of polymer chains: 4
• Total formula weight: 55487.85

Authors

Dolot, R.M.,Seda, A.,Nawrot, B. (deposition date: 2015-04-30, release date: 2016-03-02, Last modification date: 2024-01-10)

Primary citation

Dolot, R.,Kaczmarek, R.,Seda, A.,Krakowiak, A.,Baraniak, J.,Nawrot, B.
Crystallographic studies of the complex of human HINT1 protein with a non-hydrolyzable analog of Ap4A.
Int.J.Biol.Macromol., 87:62-69, 2016

PubMed Abstract: Histidine triad nucleotide-binding protein 1 (HINT1) represents the most ancient and widespread branch in the histidine triad proteins superfamily. HINT1 plays an important role in various biological processes, and it has been found in many species. Here, we report the first structure (at a 2.34Å resolution) of a complex of human HINT1 with a non-hydrolyzable analog of an Ap4A dinucleotide, containing bis-phosphorothioated glycerol mimicking a polyphosphate chain, obtained from a primitive monoclinic space group P21 crystal. In addition, the apo form of hHINT1 at the space group P21 refined to 1.92Å is reported for comparative studies.

PubMed: 26905466
DOI: 10.1016/j.ijbiomac.2016.02.047

Experimental method

X-RAY DIFFRACTION (1.92 Å)