4ZKK

The novel double-fold structure of d(GCATGCATGC)

4ZKK の概要

• エントリーDOI: 10.2210/pdb4zkk/pdb
• 分子名称: DNA (5'-D(*GP*CP*AP*TP*GP*CP*AP*TP*GP*C)-3'), COBALT (II) ION (3 entities in total)
• 機能のキーワード: folded, minor-groove tetrad, bi-loop, triplet, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3221.80

構造登録者

Thirugnanasambandam, A.,Karthik, S.,Mandal, P.K.,Gautham, N. (登録日: 2015-04-30, 公開日: 2015-10-14, 最終更新日: 2024-03-20)

主引用文献

Thirugnanasambandam, A.,Karthik, S.,Mandal, P.K.,Gautham, N.
The novel double-folded structure of d(GCATGCATGC): a possible model for triplet-repeat sequences
Acta Crystallogr.,Sect.D, 71:2119-2126, 2015

PubMed Abstract: The structure of the decadeoxyribonucleotide d(GCATGCATGC) is presented at a resolution of 1.8 Å. The decamer adopts a novel double-folded structure in which the direction of progression of the backbone changes at the two thymine residues. Intra-strand stacking interactions (including an interaction between the endocylic O atom of a ribose moiety and the adjacent purine base), hydrogen bonds and cobalt-ion interactions stabilize the double-folded structure of the single strand. Two such double-folded strands come together in the crystal to form a dimer. Inter-strand Watson-Crick hydrogen bonds form four base pairs. This portion of the decamer structure is similar to that observed in other previously reported oligonucleotide structures and has been dubbed a `bi-loop'. Both the double-folded single-strand structure, as well as the dimeric bi-loop structure, serve as starting points to construct models for triplet-repeat DNA sequences, which have been implicated in many human diseases.

PubMed: 26457435
DOI: 10.1107/S1399004715013930

実験手法

X-RAY DIFFRACTION (1.801 Å)