4ZKJ

Crystal structure of CRISPR-associated protein

Summary for 4ZKJ

• Entry DOI: 10.2210/pdb4zkj/pdb
• Descriptor: CRISPR-associated protein Cas1, GLYCEROL (3 entities in total)
• Functional Keywords: crispr, cas, unknown function
• Biological source: Streptococcus pyogenes M1 476
• Total number of polymer chains: 2
• Total formula weight: 68727.45

Authors

Ka, D.,Bae, E. (deposition date: 2015-04-30, release date: 2016-01-13, Last modification date: 2016-01-20)

Primary citation

Ka, D.,Lee, H.,Jung, Y.D.,Kim, K.,Seok, C.,Suh, N.,Bae, E.
Crystal Structure of Streptococcus pyogenes Cas1 and Its Interaction with Csn2 in the Type II CRISPR-Cas System
Structure, 24:70-79, 2016

PubMed Abstract: CRISPRs and Cas proteins constitute an RNA-guided microbial immune system against invading nucleic acids. Cas1 is a universal Cas protein found in all three types of CRISPR-Cas systems, and its role is implicated in new spacer acquisition during CRISPR-mediated adaptive immunity. Here, we report the crystal structure of Streptococcus pyogenes Cas1 (SpCas1) in a type II CRISPR-Cas system and characterize its interaction with S. pyogenes Csn2 (SpCsn2). The SpCas1 structure reveals a unique conformational state distinct from type I Cas1 structures, resulting in a more extensive dimerization interface, a more globular overall structure, and a disruption of potential metal-binding sites for catalysis. We demonstrate that SpCas1 directly interacts with SpCsn2, and identify the binding interface and key residues for Cas complex formation. These results provide structural information for a type II Cas1 protein, and lay a foundation for studying multiprotein Cas complexes functioning in type II CRISPR-Cas systems.

PubMed: 26671707
DOI: 10.1016/j.str.2015.10.019

Experimental method

X-RAY DIFFRACTION (2.25 Å)