4ZIZ

Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic Phase (C-Phycocyanin from T. elongatus)

Summary for 4ZIZ

• Entry DOI: 10.2210/pdb4ziz/pdb
• Descriptor: C-phycocyanin alpha chain, C-phycocyanin beta chain, PHYCOCYANOBILIN, ... (4 entities in total)
• Functional Keywords: free electron laser, phycocyanin, lipidic cubic phase, electron transport
• Biological source: Thermosynechococcus elongatus (strain BP-1); More
• Cellular location: Cellular thylakoid membrane ; Peripheral membrane protein ; Cytoplasmic side : P50032 P50033
• Total number of polymer chains: 2
• Total formula weight: 37439.36

Authors

Fromme, R.,Ishchenko, A.,Metz, M.,Roy-Chowdhury, S.,Basu, S.,Boutet, S.,Fromme, P.,White, T.A.,Barty, A.,Spence, J.C.H.,Weierstall, U.,Liu, W.,Cherezov, V. (deposition date: 2015-04-28, release date: 2015-08-19, Last modification date: 2024-03-13)

Primary citation

Fromme, R.,Ishchenko, A.,Metz, M.,Chowdhury, S.R.,Basu, S.,Boutet, S.,Fromme, P.,White, T.A.,Barty, A.,Spence, J.C.,Weierstall, U.,Liu, W.,Cherezov, V.
Serial femtosecond crystallography of soluble proteins in lipidic cubic phase.
IUCrJ, 2:545-551, 2015

PubMed Abstract: Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP-SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP-SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.

PubMed: 26306196
DOI: 10.1107/S2052252515013160

Experimental method

X-RAY DIFFRACTION (1.75 Å)