4ZGL

Hit Like Protein

「4Q61」から置き換えられました

4ZGL の概要

• エントリーDOI: 10.2210/pdb4zgl/pdb
• 分子名称: Uncharacterized HIT-like protein HP_0404, ADENOSINE MONOPHOSPHATE (2 entities in total)
• 機能のキーワード: cell cycle
• 由来する生物種: Helicobacter pylori 26695
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 131166.31

構造登録者

Tarique, K.F.,Devi, S.,Abdul Rehman, S.A.,Gourinath, S. (登録日: 2015-04-23, 公開日: 2015-05-27, 最終更新日: 2023-11-08)

主引用文献

Tarique, K.F.,Devi, S.,Abdul Rehman, S.A.,Gourinath, S.
Crystal structure of HINT from Helicobacter pylori.
Acta Crystallogr.,Sect.F, 72:42-48, 2016

PubMed Abstract: Proteins belonging to the histidine triad (HIT) superfamily bind nucleotides and use the histidine triad motif to carry out dinucleotidyl hydrolase, nucleotidyltransferase and phosphoramidite hydrolase activities. Five different branches of this superfamily are known to exist. Defects in these proteins in humans are linked to many diseases such as ataxia, diseases of RNA metabolism and cell-cycle regulation, and various types of cancer. The histidine triad nucleotide protein (HINT) is nearly identical to proteins that have been classified as protein kinase C-interacting proteins (PKCIs), which also have the ability to bind and inhibit protein kinase C. The structure of HINT, which exists as a homodimer, is highly conserved from humans to bacteria and shares homology with the product of fragile histidine triad protein (FHit), a tumour suppressor gene of this superfamily. Here, the structure of HINT from Helicobacter pylori (HpHINT) in complex with AMP is reported at a resolution of 3 Å. The final model has R and Rfree values of 26 and 28%, respectively, with good electron density. Structural comparison with previously reported homologues and phylogenetic analysis shows H. pylori HINT to be the smallest among them, and suggests that it branched out separately during the course of evolution. Overall, this structure has contributed to a better understanding of this protein across the animal kingdom.

PubMed: 26750483
DOI: 10.1107/S2053230X15023316

実験手法

X-RAY DIFFRACTION (2.95 Å)