4ZEI

PBP AccA from A. tumefaciens C58 in complex with L-arabinose-2-phosphate

4ZEI の概要

• エントリーDOI: 10.2210/pdb4zei/pdb
• 関連するPDBエントリー: 4RA1 4ZE8 4ZE9 4ZEB 4ZEC 4ZED
• 分子名称: ABC transporter, substrate binding protein (Agrocinopines A and B), 2-O-phosphono-alpha-L-arabinopyranose, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: pbp, class c, transport protein
• 由来する生物種: Agrobacterium tumefaciens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57718.55

構造登録者

El Sahili, A.,Morera, S. (登録日: 2015-04-20, 公開日: 2015-08-19, 最終更新日: 2024-01-10)

主引用文献

El Sahili, A.,Li, S.Z.,Lang, J.,Virus, C.,Planamente, S.,Ahmar, M.,Guimaraes, B.G.,Aumont-Nicaise, M.,Vigouroux, A.,Soulere, L.,Reader, J.,Queneau, Y.,Faure, D.,Morera, S.
A Pyranose-2-Phosphate Motif Is Responsible for Both Antibiotic Import and Quorum-Sensing Regulation in Agrobacterium tumefaciens.
Plos Pathog., 11:e1005071-e1005071, 2015

PubMed Abstract: Periplasmic binding proteins (PBPs) in association with ABC transporters select and import a wide variety of ligands into bacterial cytoplasm. They can also take up toxic molecules, as observed in the case of the phytopathogen Agrobacterium tumefaciens strain C58. This organism contains a PBP called AccA that mediates the import of the antibiotic agrocin 84, as well as the opine agrocinopine A that acts as both a nutrient and a signalling molecule for the dissemination of virulence genes through quorum-sensing. Here, we characterized the binding mode of AccA using purified agrocin 84 and synthetic agrocinopine A by X-ray crystallography at very high resolution and performed affinity measurements. Structural and affinity analyses revealed that AccA recognizes an uncommon and specific motif, a pyranose-2-phosphate moiety which is present in both imported molecules via the L-arabinopyranose moiety in agrocinopine A and the D-glucopyranose moiety in agrocin 84. We hypothesized that AccA is a gateway allowing the import of any compound possessing a pyranose-2-phosphate motif at one end. This was structurally and functionally confirmed by experiments using four synthetic compounds: agrocinopine 3'-O-benzoate, L-arabinose-2-isopropylphosphate, L-arabinose-2-phosphate and D-glucose-2-phosphate. By combining affinity measurements and in vivo assays, we demonstrated that both L-arabinose-2-phosphate and D-glucose-2-phosphate, which are the AccF mediated degradation products of agrocinopine A and agrocin 84 respectively, interact with the master transcriptional regulator AccR and activate the quorum-sensing signal synthesis and Ti plasmid transfer in A. tumefaciens C58. Our findings shed light on the role of agrocinopine and antibiotic agrocin 84 on quorum-sensing regulation in A. tumefaciens and reveal how the PBP AccA acts as vehicle for the importation of both molecules by means of a key-recognition motif. It also opens future possibilities for the rational design of antibiotic and anti-virulence compounds against A. tumefaciens or other pathogens possessing similar PBPs.

PubMed: 26244338
DOI: 10.1371/journal.ppat.1005071

実験手法

X-RAY DIFFRACTION (2.3 Å)