4ZCJ

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA1 Cys30, HA2 Cys47 mutant

4ZCJ の概要

• エントリーDOI: 10.2210/pdb4zcj/pdb
• 分子名称: Hemagglutinin, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: influenza, hemagglutinin, glycoprotein, viral protein
• 由来する生物種: Influenza A virus (strain A/Hong Kong/1/1968 H3N2); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 169160.92

構造登録者

Lee, P.S.,Wilson, I.A. (登録日: 2015-04-16, 公開日: 2015-05-13, 最終更新日: 2024-10-30)

主引用文献

Lee, P.S.,Zhu, X.,Yu, W.,Wilson, I.A.
Design and Structure of an Engineered Disulfide-Stabilized Influenza Virus Hemagglutinin Trimer.
J.Virol., 89:7417-7420, 2015

PubMed Abstract: We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved; only minor structural changes are found in the vicinity of the additional disulfide. This platform has been applied to H1 and H3 HAs and provides prospects for design of intact, stabilized influenza virus HA immunogens.

PubMed: 25926650
DOI: 10.1128/JVI.00808-15

実験手法

X-RAY DIFFRACTION (3 Å)