4ZBX

Family 4 uracil-DNA glycosylase from Sulfolobus tokodaii (free form, X-ray wavelength=0.9000)

4ZBX の概要

• エントリーDOI: 10.2210/pdb4zbx/pdb
• 関連するPDBエントリー: 4ZBY 4ZBZ
• 分子名称: Uracil-DNA glycosylase, IRON/SULFUR CLUSTER, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (4 entities in total)
• 機能のキーワード: uracil-dna glycosylase, dna repair, hydrolase
• 由来する生物種: Sulfolobus tokodaii str. 7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22906.91

構造登録者

Kawai, A.,Miyamoto, S. (登録日: 2015-04-15, 公開日: 2015-09-09, 最終更新日: 2023-11-08)

主引用文献

Kawai, A.,Higuchi, S.,Tsunoda, M.,Nakamura, K.T.,Yamagata, Y.,Miyamoto, S.
Crystal structure of family 4 uracil-DNA glycosylase from Sulfolobus tokodaii and a function of tyrosine 170 in DNA binding
Febs Lett., 589:2675-2682, 2015

PubMed Abstract: Uracil-DNA glycosylases (UDGs) excise uracil from DNA by catalyzing the N-glycosidic bond hydrolysis. Here we report the first crystal structures of an archaeal UDG (stoUDG). Compared with other UDGs, stoUDG has a different structure of the leucine-intercalation loop, which is important for DNA binding. The stoUDG-DNA complex model indicated that Leu169, Tyr170, and Asn171 in the loop are involved in DNA intercalation. Mutational analysis showed that Tyr170 is critical for substrate DNA recognition. These results indicate that Tyr170 occupies the intercalation site formed after the structural change of the leucine-intercalation loop required for the catalysis.

PubMed: 26318717
DOI: 10.1016/j.febslet.2015.08.019

実験手法

X-RAY DIFFRACTION (1.7 Å)