4ZB2

A native form of glucose isomerase collected at room temperature.

4ZB2 の概要

• エントリーDOI: 10.2210/pdb4zb2/pdb
• 関連するPDBエントリー: 4ZB5 4ZBC 4ZBO
• 分子名称: Xylose isomerase, MANGANESE (II) ION, alpha-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: isomerase
• 由来する生物種: Streptomyces rubiginosus
• 細胞内の位置: Cytoplasm: P24300
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43753.49

構造登録者

Sandy, J. (登録日: 2015-04-14, 公開日: 2016-03-23, 最終更新日: 2024-01-10)

主引用文献

Lobley, C.M.,Sandy, J.,Sanchez-Weatherby, J.,Mazzorana, M.,Krojer, T.,Nowak, R.P.,Sorensen, T.L.
A generic protocol for protein crystal dehydration using the HC1b humidity controller.
Acta Crystallogr D Struct Biol, 72:629-640, 2016

PubMed Abstract: Dehydration may change the crystal lattice and affect the mosaicity, resolution and quality of X-ray diffraction data. A dehydrating environment can be generated around a crystal in several ways with various degrees of precision and complexity. This study uses a high-precision crystal humidifier/dehumidifier to provide an airstream of known relative humidity in which the crystals are mounted: a precise yet hassle-free approach to altering crystal hydration. A protocol is introduced to assess the impact of crystal dehydration systematically applied to nine experimental crystal systems. In one case, that of glucose isomerase, dehydration triggering a change of space group from I222 to P21212 was observed. This observation is supported by an extended study of the behaviour of the glucose isomerase crystal structure during crystal dehydration.

PubMed: 27139626
DOI: 10.1107/S2059798316003065

実験手法

X-RAY DIFFRACTION (2 Å)