4Z9K

Ricin A chain bound to camelid nanobody (VHH2)(F5)

4Z9K の概要

• エントリーDOI: 10.2210/pdb4z9k/pdb
• 関連するPDBエントリー: 4LGP 4LGR 4LGS 4LHJ 4LHQ 5BOZ
• 分子名称: Ricin, VHH2(F5) antibody, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: ricin toxin, nanobodies, hydrolase-immune system complex, hydrolase/immune system
• 由来する生物種: Ricinus communis (Castor bean); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41616.01

構造登録者

Rudolph, M.J. (登録日: 2015-04-10, 公開日: 2016-07-20, 最終更新日: 2024-11-13)

主引用文献

Rudolph, M.J.,Vance, D.J.,Cassidy, M.S.,Rong, Y.,Shoemaker, C.B.,Mantis, N.J.
Structural analysis of nested neutralizing and non-neutralizing B cell epitopes on ricin toxin's enzymatic subunit.
Proteins, 84:1162-1172, 2016

PubMed Abstract: In this report, we describe the X-ray crystal structures of two single domain camelid antibodies (VH H), F5 and F8, each in complex with ricin toxin's enzymatic subunit (RTA). F5 has potent toxin-neutralizing activity, while F8 has weak neutralizing activity. F5 buried a total of 1760 Å(2) in complex with RTA and made contact with three prominent secondary structural elements: α-helix B (Residues 98-106), β-strand h (Residues 113-117), and the C-terminus of α-helix D (Residues 154-156). F8 buried 1103 Å(2) in complex with RTA that was centered primarily on β-strand h. As such, the structural epitope of F8 is essentially nested within that of F5. All three of the F5 complementarity determining regions CDRs were involved in RTA contact, whereas F8 interactions were almost entirely mediated by CDR3, which essentially formed a seventh β-strand within RTA's centrally located β-sheet. A comparison of the two structures reported here to several previously reported (RTA-VH H) structures identifies putative contact sites on RTA, particularly α-helix B, associated with potent toxin-neutralizing activity. This information has implications for rational design of RTA-based subunit vaccines for biodefense. Proteins 2016; 84:1162-1172. © 2016 Wiley Periodicals, Inc.

PubMed: 27159829
DOI: 10.1002/prot.25062

実験手法

X-RAY DIFFRACTION (1.5 Å)