4Z8V

CRYSTAL STRUCTURE OF AVRRXO1-ORF1:-ORF2 COMPLEX, NATIVE.

4Z8V の概要

• エントリーDOI: 10.2210/pdb4z8v/pdb
• 関連するPDBエントリー: 4Z8Q 4Z8T 4Z8U
• 分子名称: AvrRxo1-ORF1, AvrRxo1-ORF2, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: type iii effector proteins, chaperone, protein binding
• 由来する生物種: Xanthomonas oryzae pv. oryzicola; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48156.56

構造登録者

Han, Q.,Zhou, C.,Wu, S.,Liu, Y.,Yang, Z.,Miao, J.,Triplett, L.,Cheng, Q.,Tokuhisa, J.,Deblais, L.,Robinson, H.,Leach, J.E.,Li, J.,Zhao, B. (登録日: 2015-04-09, 公開日: 2015-09-23, 最終更新日: 2023-09-27)

主引用文献

Han, Q.,Zhou, C.,Wu, S.,Liu, Y.,Triplett, L.,Miao, J.,Tokuhisa, J.,Deblais, L.,Robinson, H.,Leach, J.E.,Li, J.,Zhao, B.
Crystal Structure of Xanthomonas AvrRxo1-ORF1, a Type III Effector with a Polynucleotide Kinase Domain, and Its Interactor AvrRxo1-ORF2.
Structure, 23:1900-1909, 2015

PubMed Abstract: Xanthomonas oryzae pv. oryzicola (Xoc) causes bacterial leaf streak (BLS) disease on rice plants. Xoc delivers a type III effector AvrRxo1-ORF1 into rice plant cells that can be recognized by disease resistance (R) protein Rxo1, and triggers resistance to BLS disease. However, the mechanism and virulence role of AvrRxo1 is not known. In the genome of Xoc, AvrRxo1-ORF1 is adjacent to another gene AvrRxo1-ORF2, which was predicted to encode a molecular chaperone of AvrRxo1-ORF1. We report the co-purification and crystallization of the AvrRxo1-ORF1:AvrRxo1-ORF2 tetramer complex at 1.64 Å resolution. AvrRxo1-ORF1 has a T4 polynucleotide kinase domain, and expression of AvrRxo1-ORF1 suppresses bacterial growth in a manner dependent on the kinase motif. Although AvrRxo1-ORF2 binds AvrRxo1-ORF1, it is structurally different from typical effector-binding chaperones, in that it has a distinct fold containing a novel kinase-binding domain. AvrRxo1-ORF2 functions to suppress the bacteriostatic activity of AvrRxo1-ORF1 in bacterial cells.

PubMed: 26344722
DOI: 10.1016/j.str.2015.06.030

実験手法

X-RAY DIFFRACTION (2.3 Å)