4Z4K

Crystal structure of GFP-TAX1BP1 UBZ1+2 domain fusion protein

Summary for 4Z4K

• Entry DOI: 10.2210/pdb4z4k/pdb
• Related: 4Z4M
• Descriptor: Green fluorescent protein,Tax1-binding protein 1, ZINC ION (3 entities in total)
• Functional Keywords: gfp, tax1bp1, ubz, zinc-finger, flurorescent protein, metal binding protein
• Biological source: Aequorea victoria (Jellyfish); More
• Total number of polymer chains: 2
• Total formula weight: 66764.35

Authors

Rohaim, A.,Kawasaki, M.,Wakatsuki, S. (deposition date: 2015-04-02, release date: 2016-04-06, Last modification date: 2024-11-06)

Primary citation

Suzuki, N.,Rohaim, A.,Kato, R.,Dikic, I.,Wakatsuki, S.,Kawasaki, M.
A novel mode of ubiquitin recognition by the ubiquitin-binding zinc finger domain of WRNIP1.
Febs J., 283:2004-2017, 2016

PubMed Abstract: The ubiquitin-binding zinc finger (UBZ) is a type of zinc-coordinating β-β-α fold domain found mainly in proteins involved in DNA repair and transcriptional regulation. Here, we report the crystal structure of the UBZ domain of Y-family DNA polymerase (pol) η and the crystal structure of the complex between the UBZ domain of Werner helicase-interacting protein 1 (WRNIP1) and ubiquitin, crystallized using the GFP fusion technique. In contrast to the pol η UBZ, which has been proposed to bind ubiquitin via its C-terminal α-helix, ubiquitin binds to a novel surface of WRNIP1 UBZ composed of the first β-strand and the C-terminal α-helix. In addition, we report the structure of the tandem UBZ domains of Tax1-binding protein 1 (TAX1BP1) and show that the second UBZ of TAX1BP1 binds ubiquitin, presumably in a manner similar to that of WRNIP1 UBZ. We propose that UBZ domains can be divided into at least two different types in terms of the ubiquitin-binding surfaces: the pol η type and the WRNIP1 type.

PubMed: 27062441
DOI: 10.1111/febs.13734

Experimental method

X-RAY DIFFRACTION (2.8 Å)