4Z2S

The crystal structure of Sclerotium Rolfsii lectin variant 2 (SSR2) in complex with N-acetyl-glucosamine

4Z2S の概要

• エントリーDOI: 10.2210/pdb4z2s/pdb
• 分子名称: Sclerotium Rolfsii lectin variant, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, 2-acetamido-2-deoxy-alpha-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: lectin, sugar binding protein, carbohydrate- binding specificity
• 由来する生物種: Athelia rolfsii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33480.92

構造登録者

Kantsadi, A.L.,Peppa, V.I.,Leonidas, D.D. (登録日: 2015-03-30, 公開日: 2015-07-01, 最終更新日: 2024-01-10)

主引用文献

Peppa, V.I.,Venkat, H.,Kantsadi, A.L.,Inamdar, S.R.,Bhat, G.G.,Eligar, S.,Shivanand, A.,Chachadi, V.B.,Satisha, G.J.,Swamy, B.M.,Skamnaki, V.T.,Zographos, S.E.,Leonidas, D.D.
Molecular Cloning, Carbohydrate Specificity and the Crystal Structure of Two Sclerotium rolfsii Lectin Variants.
Molecules, 20:10848-10865, 2015

PubMed Abstract: SRL is a cell wall associated developmental-stage specific lectin secreted by Sclerotium rolfsii, a soil-born pathogenic fungus. SRL displays specificity for TF antigen (Galβ1→3GalNAc-α-Ser//Thr) expressed in all cancer types and has tumour suppressing effects in vivo. Considering the immense potential of SRL in cancer research, we have generated two variant gene constructs of SRL and expressed in E. coli to refine the sugar specificity and solubility by altering the surface charge. SSR1 and SSR2 are two different recombinant variants of SRL, both of which recognize TF antigen but only SSR1 binds to Tn antigen (GalNAcα-Ser/Thr). The glycan array analysis of the variants demonstrated that SSR1 recognizes TF antigen and their derivative with high affinity similar to SRL but showed highest affinity towards the sialylated Tn antigen, unlike SRL. The carbohydrate binding property of SSR2 remains unaltered compared to SRL. The crystal structures of the two variants were determined in free form and in complex with N-acetylglucosamine at 1.7 Å and 1.6 Å resolution, respectively. Structural analysis highlighted the structural basis of the fine carbohydrate specificity of the two SRL variants and results are in agreement with glycan array analysis.

PubMed: 26076107
DOI: 10.3390/molecules200610848

実験手法

X-RAY DIFFRACTION (1.7 Å)