4YYC

Crystal structure of trimethylamine methyltransferase from Sinorhizobium meliloti in complex with unknown ligand

4YYC の概要

• エントリーDOI: 10.2210/pdb4yyc/pdb
• 分子名称: Putative trimethylamine methyltransferase, UNKNOWN LIGAND, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: new york structural genomics research consortium, nysgrc, trimethylamine methyltransferase, unknown ligand, psi-biology, transferase
• 由来する生物種: Rhizobium meliloti (Ensifer meliloti)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57907.03

構造登録者

Shabalin, I.G.,Porebski, P.J.,Gasiorowska, O.A.,Handing, K.B.,Niedzialkowska, E.,Cymborowski, M.T.,Cooper, D.R.,Stead, M.,Hammonds, J.,Ahmed, M.,Bonanno, J.,Seidel, R.,Almo, S.C.,Minor, W.,New York Structural Genomics Research Consortium (NYSGRC) (登録日: 2015-03-23, 公開日: 2015-04-08, 最終更新日: 2024-11-13)

主引用文献

Niedzialkowska, E.,Gasiorowska, O.,Handing, K.B.,Majorek, K.A.,Porebski, P.J.,Shabalin, I.G.,Zasadzinska, E.,Cymborowski, M.,Minor, W.
Protein purification and crystallization artifacts: The tale usually not told.
Protein Sci., 25:720-733, 2016

PubMed Abstract: The misidentification of a protein sample, or contamination of a sample with the wrong protein, may be a potential reason for the non-reproducibility of experiments. This problem may occur in the process of heterologous overexpression and purification of recombinant proteins, as well as purification of proteins from natural sources. If the contaminated or misidentified sample is used for crystallization, in many cases the problem may not be detected until structures are determined. In the case of functional studies, the problem may not be detected for years. Here several procedures that can be successfully used for the identification of crystallized protein contaminants, including: (i) a lattice parameter search against known structures, (ii) sequence or fold identification from partially built models, and (iii) molecular replacement with common contaminants as search templates have been presented. A list of common contaminant structures to be used as alternative search models was provided. These methods were used to identify four cases of purification and crystallization artifacts. This report provides troubleshooting pointers for researchers facing difficulties in phasing or model building.

PubMed: 26660914
DOI: 10.1002/pro.2861

実験手法

X-RAY DIFFRACTION (1.56 Å)