4YXA

Complex of SpaO(SPOA1,2 SeMet) and OrgB(APAR)::T4lysozyme fusion protein

Summary for 4YXA

• Entry DOI: 10.2210/pdb4yxa/pdb
• Descriptor: Surface presentation of antigens protein SpaO, Oxygen-regulated invasion protein OrgB,Endolysin, ... (4 entities in total)
• Functional Keywords: type iii secretion system, protein transport
• Biological source: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720); More
• Cellular location: Host cytoplasm : P00720
• Total number of polymer chains: 6
• Total formula weight: 76521.27

Authors

Notti, R.Q.,Stebbins, C.E. (deposition date: 2015-03-22, release date: 2015-06-03, Last modification date: 2024-10-23)

Primary citation

Notti, R.Q.,Bhattacharya, S.,Lilic, M.,Stebbins, C.E.
A common assembly module in injectisome and flagellar type III secretion sorting platforms.
Nat Commun, 6:7125-7125, 2015

PubMed Abstract: Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both systems share related elements of a cytoplasmic sorting platform that facilitates the hierarchical secretion of protein substrates, its assembly and regulation remain unclear. Here we describe a module mediating the assembly of the sorting platform in both secretion systems, and elucidate the structural basis for segregation of homologous components among these divergent T3SS subtypes sharing a common cytoplasmic milieu. These results provide a foundation for the subtype-specific assembly of T3SS sorting platforms and will support further mechanistic analysis and anti-virulence drug design.

PubMed: 25994170
DOI: 10.1038/ncomms8125

Experimental method

X-RAY DIFFRACTION (2.35 Å)