4YOP

CRYSTAL STRUCTURE OF HEN EGG-WHITE LYSOZYME

Summary for 4YOP

Entry DOI	10.2210/pdb4yop/pdb
Descriptor	Lysozyme C, SODIUM ION, CHLORIDE ION, ... (4 entities in total)
Functional Keywords	serial femtosecond crystallography, hydrolase
Biological source	Gallus gallus (Chicken)
Cellular location	Secreted: P00698
Total number of polymer chains	1
Total formula weight	14389.60
Authors	Sugahara, M.,Nakane, T.,Suzuki, M.,Nango, E. (deposition date: 2015-03-12, release date: 2015-12-23, Last modification date: 2024-10-09)
Primary citation	Nakane, T.,Song, C.,Suzuki, M.,Nango, E.,Kobayashi, J.,Masuda, T.,Inoue, S.,Mizohata, E.,Nakatsu, T.,Tanaka, T.,Tanaka, R.,Shimamura, T.,Tono, K.,Joti, Y.,Kameshima, T.,Hatsui, T.,Yabashi, M.,Nureki, O.,Iwata, S.,Sugahara, M. Native sulfur/chlorine SAD phasing for serial femtosecond crystallography Acta Crystallogr.,Sect.D, 71:2519-2525, 2015 Cited by PubMed Abstract: Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native crystal structures. PubMed: 26627659 DOI: 10.1107/S139900471501857X PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.1 Å)

Structure validation