4YOP

CRYSTAL STRUCTURE OF HEN EGG-WHITE LYSOZYME

4YOP の概要

• エントリーDOI: 10.2210/pdb4yop/pdb
• 分子名称: Lysozyme C, SODIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: serial femtosecond crystallography, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14389.60

構造登録者

Sugahara, M.,Nakane, T.,Suzuki, M.,Nango, E. (登録日: 2015-03-12, 公開日: 2015-12-23, 最終更新日: 2024-10-09)

主引用文献

Nakane, T.,Song, C.,Suzuki, M.,Nango, E.,Kobayashi, J.,Masuda, T.,Inoue, S.,Mizohata, E.,Nakatsu, T.,Tanaka, T.,Tanaka, R.,Shimamura, T.,Tono, K.,Joti, Y.,Kameshima, T.,Hatsui, T.,Yabashi, M.,Nureki, O.,Iwata, S.,Sugahara, M.
Native sulfur/chlorine SAD phasing for serial femtosecond crystallography
Acta Crystallogr.,Sect.D, 71:2519-2525, 2015

PubMed Abstract: Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native crystal structures.

PubMed: 26627659
DOI: 10.1107/S139900471501857X

実験手法

X-RAY DIFFRACTION (2.1 Å)