4YNA

Oxidized YfiR

4YNA の概要

• エントリーDOI: 10.2210/pdb4yna/pdb
• 関連するPDBエントリー: 4YN7 4YN9
• 分子名称: YfiR, SULFATE ION (2 entities in total)
• 機能のキーワード: periplasmic repressor protein, oxidized form, transcription
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 69763.26

構造登録者

Xu, M.,Jiang, T. (登録日: 2015-03-09, 公開日: 2015-04-22, 最終更新日: 2024-10-23)

主引用文献

Yang, X.,Yang, X.-A.,Xu, M.,Zhou, L.,Fan, Z.,Jiang, T.
Crystal structures of YfiR from Pseudomonas aeruginosa in two redox states
Biochem.Biophys.Res.Commun., 461:14-20, 2015

PubMed Abstract: YfiBNR is a recently identified c-di-GMP regulatory system involved in bacterial biofilm formation. The periplasmic protein YfiR inhibits the diguanylate cyclase activity of the inner membrane protein YfiN, whereas YfiB in the outer membrane can release this inhibition by sequestration of YfiR. In addition, this system may respond to anoxic conditions via YfiR, although the detailed mechanism is still unknown. Here we report crystal structures of Pseudomonas aeruginosa YfiR in the absence and presence of oxidative glutathione. Our structures reveal the overall folding of YfiR for the first time and demonstrate that YfiR exist as a dimer. Comparison of the two structures in different redox states revealed a broken/formation of one disulfide bond (Cys71-Cys110) and local conformational change around the other one (Cys145-Cys152). Mutagenesis studies indicated that Cys145-Cys152 plays an important role in maintaining the correct folding of YfiR.

PubMed: 25849887
DOI: 10.1016/j.bbrc.2015.03.160

実験手法

X-RAY DIFFRACTION (3.2 Å)