4YN9

YfiR mutant-C110S

Summary for 4YN9

• Entry DOI: 10.2210/pdb4yn9/pdb
• Related: 4YN7 4YNA
• Descriptor: YfiR, SULFATE ION (3 entities in total)
• Functional Keywords: periplasmic repressor protein, c110s mutant, transcription
• Biological source: Pseudomonas aeruginosa PAO1
• Total number of polymer chains: 2
• Total formula weight: 34945.56

Authors

Xu, M.,Jiang, T. (deposition date: 2015-03-09, release date: 2015-04-22, Last modification date: 2024-10-16)

Primary citation

Yang, X.,Yang, X.-A.,Xu, M.,Zhou, L.,Fan, Z.,Jiang, T.
Crystal structures of YfiR from Pseudomonas aeruginosa in two redox states
Biochem.Biophys.Res.Commun., 461:14-20, 2015

PubMed Abstract: YfiBNR is a recently identified c-di-GMP regulatory system involved in bacterial biofilm formation. The periplasmic protein YfiR inhibits the diguanylate cyclase activity of the inner membrane protein YfiN, whereas YfiB in the outer membrane can release this inhibition by sequestration of YfiR. In addition, this system may respond to anoxic conditions via YfiR, although the detailed mechanism is still unknown. Here we report crystal structures of Pseudomonas aeruginosa YfiR in the absence and presence of oxidative glutathione. Our structures reveal the overall folding of YfiR for the first time and demonstrate that YfiR exist as a dimer. Comparison of the two structures in different redox states revealed a broken/formation of one disulfide bond (Cys71-Cys110) and local conformational change around the other one (Cys145-Cys152). Mutagenesis studies indicated that Cys145-Cys152 plays an important role in maintaining the correct folding of YfiR.

PubMed: 25849887
DOI: 10.1016/j.bbrc.2015.03.160

Experimental method

X-RAY DIFFRACTION (2.45 Å)