4YKO

Crystal structure of the R111K:Y134F:T54V:R132Q:P39Q:R59Y:A32Y:F3Q mutant of human Cellular Retinoic Acid Binding Protein II with Retinal at 1.58 angstrom resolution

4YKO の概要

• エントリーDOI: 10.2210/pdb4yko/pdb
• 関連するPDBエントリー: 2G7B 4EEJ 4I9S 4RUU 4YFP 4YFQ 4YFR
• 分子名称: Cellular retinoic acid-binding protein 2, RETINAL (3 entities in total)
• 機能のキーワード: rhodopsin mimic, photoswitchable protein, retinal isomerization, retinal psb, protein engineering, retinal iminium pka change by isomerization, transport protein
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm: P29373
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31802.47

構造登録者

Nosrati, M.,Geiger, J. (登録日: 2015-03-04, 公開日: 2016-03-09, 最終更新日: 2024-10-23)

主引用文献

Nosrati, M.,Berbasova, T.,Vasileiou, C.,Borhan, B.,Geiger, J.H.
A Photoisomerizing Rhodopsin Mimic Observed at Atomic Resolution.
J.Am.Chem.Soc., 138:8802-8808, 2016

PubMed Abstract: The members of the rhodopsin family of proteins are involved in many essential light-dependent processes in biology. Specific photoisomerization of the protein-bound retinylidene PSB at a specified wavelength range of light is at the heart of all of these systems. Nonetheless, it has been difficult to reproduce in an engineered system. We have developed rhodopsin mimics, using intracellular lipid binding protein family members as scaffolds, to study fundamental aspects of protein/chromophore interactions. Herein we describe a system that specifically isomerizes the retinylidene protonated Schiff base both thermally and photochemically. This isomerization has been characterized at atomic resolution by quantitatively interconverting the isomers in the crystal both thermally and photochemically. This event is accompanied by a large pKa change of the imine similar to the pKa changes observed in bacteriorhodopsin and visual opsins during isomerization.

PubMed: 27310917
DOI: 10.1021/jacs.6b03681

実験手法

X-RAY DIFFRACTION (1.57 Å)