4YKB

Structure of GUN4 from Chlamydomonas reinhardtii

4YKB の概要

• エントリーDOI: 10.2210/pdb4ykb/pdb
• 分子名称: Tetrapyrrole-binding protein (1 entity in total)
• 機能のキーワード: plant protein
• 由来する生物種: Chlamydomonas reinhardtii
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 154938.25

構造登録者

Tabriz, S.T.,Langley, D.B.,Willows, R.D.,Duff, A.P.,Harrop, S.J. (登録日: 2015-03-04, 公開日: 2015-08-12, 最終更新日: 2023-09-27)

主引用文献

Tarahi Tabrizi, S.,Langley, D.B.,Harrop, S.J.,Duff, A.P.,Willows, R.D.
Structure of GUN4 from Chlamydomonas reinhardtii.
Acta Crystallogr F Struct Biol Commun, 71:1094-1099, 2015

PubMed Abstract: The genomes uncoupled 4 (GUN4) protein stimulates chlorophyll biosynthesis by increasing the activity of Mg-chelatase, the enzyme that inserts magnesium into protoporphyrin IX (PPIX) in the chlorophyll biosynthesis pathway. One of the roles of GUN4 is in binding PPIX and Mg-PPIX. In eukaryotes, GUN4 also participates in plastid-to-nucleus signalling, although the mechanism for this is unclear. Here, the first crystal structure of a eukaryotic GUN4, from Chlamydomonas reinhardtii, is presented. The structure is in broad agreement with those of previously solved cyanobacterial structures. Most interestingly, conformational divergence is restricted to several loops which cover the porphyrin-binding cleft. The conformational dynamics suggested by this ensemble of structures lend support to the understanding of how GUN4 binds PPIX or Mg-PPIX.

PubMed: 26249706
DOI: 10.1107/S2053230X15012248

実験手法

X-RAY DIFFRACTION (3.5 Å)