4YFB

Structure of N-acylhomoserine lactone acylase MacQ in complex with phenylacetic acid

4YFB の概要

• エントリーDOI: 10.2210/pdb4yfb/pdb
• 関連するPDBエントリー: 4YF9 4YFA
• 分子名称: Protein related to penicillin acylase, 2-PHENYLACETIC ACID, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: acylase, product complex, ntn-hydrolase fold, hydrolase
• 由来する生物種: Acidovorax sp. MR-S7; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 338900.38

構造登録者

Yasutake, Y.,Kusada, H.,Kimura, N. (登録日: 2015-02-25, 公開日: 2016-03-02, 最終更新日: 2023-11-08)

主引用文献

Yasutake, Y.,Kusada, H.,Ebuchi, T.,Hanada, S.,Kamagata, Y.,Tamura, T.,Kimura, N.
Bifunctional quorum-quenching and antibiotic-acylase MacQ forms a 170-kDa capsule-shaped molecule containing spacer polypeptides
Sci Rep, 7:8946-8946, 2017

PubMed Abstract: Understanding the molecular mechanisms of bacterial antibiotic resistance will help prepare against further emergence of multi-drug resistant strains. MacQ is an enzyme responsible for the multi-drug resistance of Acidovorax sp. strain MR-S7. MacQ has acylase activity against both N-acylhomoserine lactones (AHLs), a class of signalling compounds involved in quorum sensing, and β-lactam antibiotics. Thus, MacQ is crucial as a quencher of quorum sensing as well as in conferring antibiotic resistance in Acidovorax. Here, we report the X-ray structures of MacQ in ligand-free and reaction product complexes. MacQ forms a 170-kDa capsule-shaped molecule via face-to-face interaction with two heterodimers consisting of an α-chain and a β-chain, generated by the self-cleaving activity of a precursor polypeptide. The electron density of the spacer polypeptide in the hollow of the molecule revealed the close orientation of the peptide-bond atoms of Val20SP-Gly21SP to the active-site, implying a role of the residues in substrate binding. In mutational analyses, uncleaved MacQ retained degradation activity against both AHLs and penicillin G. These results provide novel insights into the mechanism of self-cleaving maturation and enzymatic function of N-terminal nucleophile hydrolases.

PubMed: 28827579
DOI: 10.1038/s41598-017-09399-4

実験手法

X-RAY DIFFRACTION (1.75 Å)