4YD9

Crystal structure of squid hemocyanin

これはPDB形式変換不可エントリーです。

4YD9 の概要

• エントリーDOI: 10.2210/pdb4yd9/pdb
• 分子名称: hemocyanin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: decamer, supermolecule, oxygen transport
• 由来する生物種: Todarodes pacificus; 詳細
• タンパク質・核酸の鎖数: 30
• 化学式量合計: 3835835.70

構造登録者

Matsuno, A.,Gai, Z.,Kato, K.,Tanaka, Y.,Yao, M. (登録日: 2015-02-21, 公開日: 2015-10-14, 最終更新日: 2025-09-17)

主引用文献

Gai, Z.,Matsuno, A.,Kato, K.,Kato, S.,Khan, M.R.I.,Shimizu, T.,Yoshioka, T.,Kato, Y.,Kishimura, H.,Kanno, G.,Miyabe, Y.,Terada, T.,Tanaka, Y.,Yao, M.
Crystal Structure of the 3.8-MDa Respiratory Supermolecule Hemocyanin at 3.0 angstrom Resolution
Structure, 23:2204-2212, 2015

PubMed Abstract: Molluscan hemocyanin, a copper-containing oxygen transporter, is one of the largest known proteins. Although molluscan hemocyanins are currently applied as immunotherapeutic agents, their precise structure has not been determined because of their enormous size. Here, we have determined the first X-ray crystal structure of intact molluscan hemocyanin. The structure unveiled the architecture of the 3.8-MDa supermolecule composed of homologous functional units (FUs), wherein the dimers of FUs hierarchically associated to form the entire cylindrical decamer. Most of the specific inter-FU interactions were localized at narrow regions in the FU dimers, suggesting that rigid FU dimers formed by specific interactions assemble with flexibility. Furthermore, the roles of carbohydrates in assembly and allosteric effect, and conserved sulfur-containing residues in copper incorporation, were revealed. The precise structural information obtained in this study will accelerate our understanding of the molecular basis of hemocyanin and its future applications.

PubMed: 26602184
DOI: 10.1016/j.str.2015.09.008

実験手法

X-RAY DIFFRACTION (3 Å)