4Y42

Cyanase (CynS) from Serratia proteamaculans

4Y42 の概要

• エントリーDOI: 10.2210/pdb4y42/pdb
• 分子名称: Cyanate hydratase, GLYCEROL (3 entities in total)
• 機能のキーワード: cyns, cyanase, lyase
• 由来する生物種: Serratia proteamaculans
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 172096.21

構造登録者

Butryn, A.,Hopfner, K.-P. (登録日: 2015-02-10, 公開日: 2015-04-08, 最終更新日: 2024-01-10)

主引用文献

Butryn, A.,Stoehr, G.,Linke-Winnebeck, C.,Hopfner, K.-P.
Serendipitous crystallization and structure determination of cyanase (CynS) from Serratia proteamaculans
Acta Crystallogr.,Sect.F, 71:471-476, 2015

PubMed Abstract: Cyanate hydratase (CynS) catalyzes the decomposition of cyanate and bicarbonate into ammonia and carbon dioxide. Here, the serendipitous crystallization of CynS from Serratia proteamaculans (SpCynS) is reported. SpCynS was crystallized as an impurity and its identity was determined using mass-spectrometric analysis. The crystals belonged to space group P1 and diffracted to 2.1 Å resolution. The overall structure of SpCynS is very similar to a previously determined structure of CynS from Escherichia coli. Density for a ligand bound to the SpCynS active site was observed, but could not be unambiguously identified. Additionally, glycerol molecules bound at the entry to the active site of the enzyme indicate conserved residues that might be important for the trafficking of substrates and products.

PubMed: 25849512
DOI: 10.1107/S2053230X15004902

実験手法

X-RAY DIFFRACTION (2.09 Å)