4Y2N

Structure of CFA/I pili major subunit CfaB trimer

4Y2N の概要

• エントリーDOI: 10.2210/pdb4y2n/pdb
• 関連するPDBエントリー: 4Y2L 4Y2O
• 分子名称: CFA/I fimbrial subunit B, MAGNESIUM ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: enterotoxigenic escherichia coli, periplasmic chaperone, major pilin, self-assembly, fimbriae, structural protein
• 由来する生物種: Escherichia coli O78:H11 (strain H10407 / ETEC)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 47969.73

構造登録者

Bao, R.,Xia, D. (登録日: 2015-02-10, 公開日: 2016-08-10, 最終更新日: 2023-09-27)

主引用文献

Bao, R.,Liu, Y.,Savarino, S.J.,Xia, D.
Off-pathway assembly of fimbria subunits is prevented by chaperone CfaA of CFA/I fimbriae from enterotoxigenic E. coli.
Mol. Microbiol., 102:975-991, 2016

PubMed Abstract: The assembly of the class 5 colonization factor antigen I (CFA/I) fimbriae of enterotoxigenic E. coli was proposed to proceed via the alternate chaperone-usher pathway. Here, we show that in the absence of the chaperone CfaA, CfaB, the major pilin subunit of CFA/I fimbriae, is able to spontaneously refold and polymerize into cyclic trimers. CfaA kinetically traps CfaB to form a metastable complex that can be stabilized by mutations. Crystal structure of the stabilized complex reveals distinctive interactions provided by CfaA to trap CfaB in an assembly competent state through donor-strand complementation (DSC) and cleft-mediated anchorage. Mutagenesis indicated that DSC controls the stability of the chaperone-subunit complex and the cleft-mediated anchorage of the subunit C-terminus additionally assist in subunit refolding. Surprisingly, over-stabilization of the chaperone-subunit complex led to delayed fimbria assembly, whereas destabilizing the complex resulted in no fimbriation. Thus, CfaA acts predominantly as a kinetic trap by stabilizing subunit to avoid its off-pathway self-polymerization that results in energetically favorable trimers and could serve as a driving force for CFA/I pilus assembly, representing an energetic landscape unique to class 5 fimbria assembly.

PubMed: 27627030
DOI: 10.1111/mmi.13530

実験手法

X-RAY DIFFRACTION (2.4 Å)