4Y1B

Structure of crotonyl-CoA carboxylase/reductase AntE V350A in complex with NADP

Summary for 4Y1B

• Entry DOI: 10.2210/pdb4y1b/pdb
• Related: 4Y0K
• Descriptor: AntE, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (3 entities in total)
• Functional Keywords: oxidoreductase, transferase, carboxylase, antimycin biosynthesis
• Biological source: Streptomyces sp. NRRL 2288
• Total number of polymer chains: 1
• Total formula weight: 47353.96

Authors

Abe, I.,Zhang, L.,Mori, T. (deposition date: 2015-02-07, release date: 2015-09-23, Last modification date: 2023-11-08)

Primary citation

Zhang, L.,Mori, T.,Zheng, Q.,Awakawa, T.,Yan, Y.,Liu, W.,Abe, I.
Rational Control of Polyketide Extender Units by Structure-Based Engineering of a Crotonyl-CoA Carboxylase/Reductase in Antimycin Biosynthesis
Angew.Chem.Int.Ed.Engl., 54:13462-13465, 2015

PubMed Abstract: Bioengineering of natural product biosynthesis is a powerful approach to expand the structural diversity of bioactive molecules. However, in polyketide biosynthesis, the modification of polyketide extender units, which form the carbon skeletons, has remained challenging. Herein, we report the rational control of polyketide extender units by the structure-based engineering of a crotonyl-CoA carboxylase/reductase (CCR), in the biosynthesis of antimycin. Site-directed mutagenesis of the CCR enzyme AntE, guided by the crystal structure solved at 1.5 Å resolution, expanded its substrate scope to afford indolylmethylmalonyl-CoA by the V350G mutation. The mutant A182L selectively catalyzed carboxylation over the regular reduction. Furthermore, the combinatorial biosynthesis of heterocycle- and substituted arene-bearing antimycins was achieved by an engineered Streptomyces strain bearing AntE(V350G). These findings deepen our understanding of the molecular mechanisms of the CCRs, which will serve as versatile biocatalysts for the manipulation of building blocks, and set the stage for the rational design of polyketide biosynthesis.

PubMed: 26356484
DOI: 10.1002/anie.201506899

Experimental method

X-RAY DIFFRACTION (1.5 Å)