4Y13

SdiA in complex with octanoyl-rac-glycerol

4Y13 の概要

• エントリーDOI: 10.2210/pdb4y13/pdb
• 関連するPDBエントリー: 4Y15 4Y17
• 分子名称: Transcriptional regulator of ftsQAZ gene cluster, SULFATE ION, (2S)-2,3-dihydroxypropyl octanoate, ... (5 entities in total)
• 機能のキーワード: quorum sensor, dna, dna binding protein
• 由来する生物種: Escherichia coli O157:H7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30134.51

構造登録者

Nguyen, N.X.,Nguyen, Y.,Sperandio, V.,Jiang, Y. (登録日: 2015-02-06, 公開日: 2015-04-08, 最終更新日: 2024-11-06)

主引用文献

Nguyen, Y.,Nguyen, N.X.,Rogers, J.L.,Liao, J.,MacMillan, J.B.,Jiang, Y.,Sperandio, V.
Structural and Mechanistic Roles of Novel Chemical Ligands on the SdiA Quorum-Sensing Transcription Regulator.
Mbio, 6:-, 2015

PubMed Abstract: Bacteria engage in chemical signaling, termed quorum sensing (QS), to mediate intercellular communication, mimicking multicellular organisms. The LuxR family of QS transcription factors regulates gene expression, coordinating population behavior by sensing endogenous acyl homoserine lactones (AHLs). However, some bacteria (such as Escherichia coli) do not produce AHLs. These LuxR orphans sense exogenous AHLs but also regulate transcription in the absence of AHLs. Importantly, this AHL-independent regulatory mechanism is still largely unknown. Here we present several structures of one such orphan LuxR-type protein, SdiA, from enterohemorrhagic E. coli (EHEC), in the presence and absence of AHL. SdiA is actually not in an apo state without AHL but is regulated by a previously unknown endogenous ligand, 1-octanoyl-rac-glycerol (OCL), which is ubiquitously found throughout the tree of life and serves as an energy source, signaling molecule, and substrate for membrane biogenesis. While exogenous AHL renders to SdiA higher stability and DNA binding affinity, OCL may function as a chemical chaperone placeholder that stabilizes SdiA, allowing for basal activity. Structural comparison between SdiA-AHL and SdiA-OCL complexes provides crucial mechanistic insights into the ligand regulation of AHL-dependent and -independent function of LuxR-type proteins. Importantly, in addition to its contribution to basic science, this work has implications for public health, inasmuch as the SdiA signaling system aids the deadly human pathogen EHEC to adapt to a commensal lifestyle in the gastrointestinal (GI) tract of cattle, its main reservoir. These studies open exciting and novel avenues to control shedding of this human pathogen in the environment.

PubMed: 25827420
DOI: 10.1128/mBio.02429-14

実験手法

X-RAY DIFFRACTION (3.096 Å)