4Y07

Crystal structure of the HECT domain of human WWP2

4Y07 の概要

• エントリーDOI: 10.2210/pdb4y07/pdb
• 分子名称: NEDD4-like E3 ubiquitin-protein ligase WWP2 (2 entities in total)
• 機能のキーワード: e3 ligase, hect domain, ligase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Nucleus : O00308
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44771.23

構造登録者

Gong, W.,Li, J.,Li, Z.,Xu, Y. (登録日: 2015-02-05, 公開日: 2015-11-04, 最終更新日: 2023-11-08)

主引用文献

Gong, W.,Zhang, X.,Zhang, W.,Li, J.,Li, Z.
Structure of the HECT domain of human WWP2
Acta Crystallogr.,Sect.F, 71:1251-1257, 2015

PubMed Abstract: WWP2 is a HECT-domain ubiquitin ligase of the Nedd4 family, which is involved in various important biological processes, such as protein degradation, membrane-protein sorting and transportation, the immune response, pluripotency of embryonic stem cells, tumourigenesis and metastasis. The HECT domain provides the intrinsic ubiquitin ligase activity of WWP2. Here, the expression, purification, crystallization and crystallographic analysis of the HECT domain of human WWP2 (HECT(WWP2)) are reported. HECT(WWP2) has been crystallized and the crystals diffracted to 2.50 Å resolution. They belonged to space group P41212 and the structure has been solved via molecular replacement. The overall structure of HECT(WWP2) has an inverted T-shape. This structure displays a high degree of conservation with previously published structures of Nedd4 subfamily members.

PubMed: 26457515
DOI: 10.1107/S2053230X1501554X

実験手法

X-RAY DIFFRACTION (2.507 Å)