4XZL

Crystal structure of human AKR1B10 complexed with NADP+ and JF0049

4XZL の概要

• エントリーDOI: 10.2210/pdb4xzl/pdb
• 関連するPDBエントリー: 1ZUA
• 分子名称: Aldo-keto reductase family 1 member B10, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, [2,4-dioxo-3-(2,3,4,5-tetrabromo-6-methoxybenzyl)-3,4-dihydropyrimidin-1(2H)-yl]acetic acid, ... (5 entities in total)
• 機能のキーワード: tim barrel, aldo-keto reductase 1b10, oxidoreductase, diabetes, halogenated compound, cytosolic
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Lysosome : O60218
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37805.36

構造登録者

Cousido-Siah, A.,Ruiz, F.X.,Mitschler, A.,Dominguez, M.,de Lera, A.R.,Farres, J.,Pares, X.,Podjarny, A. (登録日: 2015-02-04, 公開日: 2015-11-18, 最終更新日: 2024-01-10)

主引用文献

Ruiz, F.X.,Cousido-Siah, A.,Porte, S.,Dominguez, M.,Crespo, I.,Rechlin, C.,Mitschler, A.,de Lera, A.R.,Martin, M.J.,de la Fuente, J.A.,Klebe, G.,Pares, X.,Farres, J.,Podjarny, A.
Structural Determinants of the Selectivity of 3-Benzyluracil-1-acetic Acids toward Human Enzymes Aldose Reductase and AKR1B10.
Chemmedchem, 10:1989-2003, 2015

PubMed Abstract: The human enzymes aldose reductase (AR) and AKR1B10 have been thoroughly explored in terms of their roles in diabetes, inflammatory disorders, and cancer. In this study we identified two new lead compounds, 2-(3-(4-chloro-3-nitrobenzyl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetic acid (JF0048, 3) and 2-(2,4-dioxo-3-(2,3,4,5-tetrabromo-6-methoxybenzyl)-3,4-dihydropyrimidin-1(2H)-yl)acetic acid (JF0049, 4), which selectively target these enzymes. Although 3 and 4 share the 3-benzyluracil-1-acetic acid scaffold, they have different substituents in their aryl moieties. Inhibition studies along with thermodynamic and structural characterizations of both enzymes revealed that the chloronitrobenzyl moiety of compound 3 can open the AR specificity pocket but not that of the AKR1B10 cognate. In contrast, the larger atoms at the ortho and/or meta positions of compound 4 prevent the AR specificity pocket from opening due to steric hindrance and provide a tighter fit to the AKR1B10 inhibitor binding pocket, probably enhanced by the displacement of a disordered water molecule trapped in a hydrophobic subpocket, creating an enthalpic signature. Furthermore, this selectivity also occurs in the cell, which enables the development of a more efficient drug design strategy: compound 3 prevents sorbitol accumulation in human retinal ARPE-19 cells, whereas 4 stops proliferation in human lung cancer NCI-H460 cells.

PubMed: 26549844
DOI: 10.1002/cmdc.201500393

実験手法

X-RAY DIFFRACTION (1.7 Å)