4XZE

The crystal structure of Hazara virus nucleoprotein

Summary for 4XZE

• Entry DOI: 10.2210/pdb4xze/pdb
• Related: 4XZ8 4XZA 4XZC
• Descriptor: Nucleoprotein (2 entities in total)
• Functional Keywords: nucleoprotein, hazara virus, rna binding protein
• Biological source: Hazara virus
• Total number of polymer chains: 4
• Total formula weight: 218180.09

Authors

Guo, Y.,Wang, W.,Liu, X.,Wang, X.,Wang, J.,Huo, T.,Liu, B. (deposition date: 2015-02-04, release date: 2015-09-02, Last modification date: 2023-11-08)

Primary citation

Wang, W.,Liu, X.,Wang, X.,Dong, H.,Ma, C.,Wang, J.,Liu, B.,Mao, Y.,Wang, Y.,Li, T.,Yang, C.,Guo, Y.
Structural and Functional Diversity of Nairovirus-Encoded Nucleoproteins.
J.Virol., 89:11740-11749, 2015

PubMed Abstract: The nairoviruses include assorted tick-borne bunyaviruses that are emerging as causative agents of infectious diseases among humans and animals. As negative-sense single-stranded RNA (-ssRNA) viruses, nairoviruses encode nucleoprotein (NP) that encapsidates the genomic RNA and further forms ribonucleoprotein (RNP) complex with viral RNA-dependent RNA polymerase (RdRp). We previously revealed that the monomeric NP encoded by Crimean-Congo hemorrhagic fever virus (CCHFV) presents a racket-shaped structure and shows unusual DNA-specific endonuclease activity. To examine the structural and biological variation of nairovirus-encoded NPs, here, we systematically solved the crystal structures of NPs encoded by various nairoviruses, including Hazara virus (HAZV), Kupe virus (KUPV), and Erve virus (ERVEV). Combined with biochemical analysis, our results generate a clearer picture to aid in the understanding of the functional diversity of nairovirus-encoded NPs and the formation of nairovirus RNPs.

PubMed: 26246561
DOI: 10.1128/JVI.01680-15

Experimental method

X-RAY DIFFRACTION (2.9 Å)