4XUW

Structure of the hazelnut allergen, Cor a 8

4XUW の概要

• エントリーDOI: 10.2210/pdb4xuw/pdb
• 分子名称: Non-specific lipid-transfer protein, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: allergen, plant protein
• 由来する生物種: Corylus avellana (European hazel)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19068.99

構造登録者

Offermann, L.R.,Perdue, M.L.,Bublin, M.,Pfeifer, S.,Dubiela, P.,Hoffmann-Sommergruber, K.,Chruszcz, M. (登録日: 2015-01-26, 公開日: 2015-10-14, 最終更新日: 2024-10-16)

主引用文献

Offermann, L.R.,Bublin, M.,Perdue, M.L.,Pfeifer, S.,Dubiela, P.,Borowski, T.,Chruszcz, M.,Hoffmann-Sommergruber, K.
Structural and Functional Characterization of the Hazelnut Allergen Cor a 8.
J.Agric.Food Chem., 63:9150-9158, 2015

PubMed Abstract: Nonspecific lipid transfer proteins (nsLTPs) are basic proteins, stabilized by four disulfide bonds, and are expressed throughout the plant kingdom. These proteins are also known as important allergens in fruits and tree nuts. In this study, the nsLTP from hazelnuts, Cor a 8, was purified and its crystal structure determined. The protein is stable at low pH and refolds after thermal denaturation. Molecular dynamics simulations were used to provide an insight into conformational changes of Cor a 8 upon ligand binding. When known epitope areas from Pru p 3 were compared to those of Cor a 8, differences were obvious, which may contribute to limited cross-reactivity between peach and hazelnut allergens. Differences in epitope regions may contribute to limited cross-reactivity between Cor a 8 and nsLTPs from other plant sources. The structure of Cor a 8 represents the first resolved structure of a hazelnut allergen.

PubMed: 26417906
DOI: 10.1021/acs.jafc.5b03534

実験手法

X-RAY DIFFRACTION (1.1 Å)